Human Microsomal Triglyceride Transfer Protein (MTTP) ELISA Kit | MTTP elisa kit

Human Microsomal Triglyceride Transfer Protein (MTTP) ELISA Kit

Cat. Num. AAA457080

• Gene Names: MTTP; ABL; MTP
• Reactivity: Human
• Synonyms: Microsomal Triglyceride Transfer Protein (MTTP); Human Microsomal Triglyceride Transfer Protein (MTTP) ELISA Kit; MTP; ABL; Microsomal triglyceride transfer protein large subunit; MTTP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MTTP. No significant cross-reactivity or interference between MTTP and analogues was observed.
• Sequence Length: 894

• Samples: Tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: <36pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MTTP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MTTP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTTP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MTTP in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MTTP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MTTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MTTP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MTTP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 153285408
• NCBI GeneID: 4547
• NCBI Accession #: NP_000244.2
• NCBI GenBank Nucleotide #: NM_000253.3
• UniProt Accession #: P55157; Q08AM4; Q6P5T3; A8K428
• Molecular Weight: 16,865 Da
• NCBI Official Full Name: microsomal triglyceride transfer protein large subunit isoform 1
• NCBI Official Synonym Full Names: microsomal triglyceride transfer protein
• NCBI Official Symbol: MTTP
• NCBI Official Synonym Symbols: ABL; MTP
• NCBI Protein Information: microsomal triglyceride transfer protein large subunit
• UniProt Protein Name: Microsomal triglyceride transfer protein large subunit
• Protein Family: Microsomal triglyceride transfer protein
• UniProt Gene Name: MTTP
• UniProt Synonym Gene Names: MTP

NCBI Description

MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces (PubMed:23475612, PubMed:8939939, PubMed:26224785, PubMed:25108285, PubMed:22236406). Required for the secretion of plasma lipoproteins that contain apolipoprotein B (PubMed:23475612, PubMed:8939939, PubMed:26224785).