Rabbit anti-Human, Mouse BAIAP2 Polyclonal Antibody | anti-BAIAP2 antibody

BAIAP2, CT (Brain-specific Angiogenesis Inhibitor 1-associated Protein 2, BAI1-associated Protein 2, BAI-associated Protein 2, Protein BAP2, Insulin Receptor Substrate Protein Of 53kD, Insulin Receptor Substrate p53, IRSp53, Insulin Receptor Substrate p53

Cat. Num. AAA6464858

• Gene Names: BAIAP2; BAP2; FLAF3; IRSP53
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: BAIAP2; Polyclonal Antibody; CT (Brain-specific Angiogenesis Inhibitor 1-associated Protein 2; BAI1-associated Protein 2; BAI-associated Protein 2; Protein BAP2; Insulin Receptor Substrate Protein Of 53kD; Insulin Receptor Substrate p53; IRSp53; anti-BAIAP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BAIAP2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-BAIAP2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human BAIAP2 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-BAIAP2 Pab is used in Western blot to detect BAIAP2 in mouse brain tissue lysate (Lane 1) and A375 cell lysate (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-BAIAP2 antibody

Antibodies; Neuroscience

References

1. Fujiwara, T., et al., Biochem. Biophys. Res. Commun. 271(3):626-629 (2000). 2. Abbott, M.A., et al., J. Neurosci. 19(17):7300-7308 (1999). 3. Okamura-Oho, Y., et al., Hum. Mol. Genet. 8(6):947-957 (1999). 4. Oda, K., et al., Cytogenet. Cell Genet. 84(1-2), 75-82 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 5453565
• NCBI GeneID: 10458
• NCBI Accession #: NP_006331
• NCBI GenBank Nucleotide #: NM_006340.2
• UniProt Accession #: Q9UQB8; O43858; Q53HB1; Q86WC1; Q8N5C0; Q96CR7; Q9UBR3; Q9UQ43
• Molecular Weight: 57,430 Da
• NCBI Official Full Name: brain-specific angiogenesis inhibitor 1-associated protein 2 isoform 3
• NCBI Official Synonym Full Names: BAI1-associated protein 2
• NCBI Official Symbol: BAIAP2
• NCBI Official Synonym Symbols: BAP2; FLAF3; IRSP53
• NCBI Protein Information: brain-specific angiogenesis inhibitor 1-associated protein 2
• UniProt Protein Name: Brain-specific angiogenesis inhibitor 1-associated protein 2
• Protein Family: Brain-specific angiogenesis inhibitor 1-associated protein
• UniProt Gene Name: BAIAP2
• UniProt Synonym Gene Names: BAI-associated protein 2; BAI1-associated protein 2; Protein BAP2; FLAF3; IRS-58; IRSp53/58; IRSp53; Insulin receptor substrate p53
• UniProt Entry Name: BAIP2_HUMAN

NCBI Description

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

IRSp53: Adapter protein that links membrane-bound small G- proteins to cytoplasmic effector proteins. Necessary for CDC42- mediated reorganization of the actin cytoskeleton and for RAC1- mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Homodimer. Interacts with CDC42 and RAC1 that have been activated by GTP binding. Interacts with ATN1, BAI1, EPS8, SHANK1, SHANK2, SHANK3, WASF1 and WASF2. Interacts with ENAH after recruitment of CDC42. Interacts with TIAM1 and DIAPH1. Interacts (via SH3 domain) with E.coli effector protein EspF(U) (via PXXP motifs). Interacts with E.coli intimin receptor Tir. Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleoplasm; ruffle; neuron projection; cytoplasm; plasma membrane; cytosol; actin cytoskeleton; filopodium

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; cytoskeletal adaptor activity

Biological Process: actin crosslink formation; regulation of cell shape; actin filament bundle formation; plasma membrane organization and biogenesis; response to bacterium; axonogenesis; regulation of actin cytoskeleton organization and biogenesis; insulin receptor signaling pathway; dendrite development; regulation of synaptic plasticity; innate immune response; vascular endothelial growth factor receptor signaling pathway