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Western Blot (WB) (Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/mL.)

Rabbit anti-Human, Mouse AES Polyclonal Antibody | anti-AES antibody

AES Antibody

Gene Names
AES; GRG; ESP1; GRG5; TLE5; AES-1; AES-2; Grg-5
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Affinity chromatography purified via peptide column
Synonyms
AES; Polyclonal Antibody; AES Antibody; Amino-terminal enhancer of split; GRG; ESP1; TLE5; anti-AES antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
264
Applicable Applications for anti-AES antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Immunogen Type
Peptide
Immunogen Description
Raised against a 16 amino acid peptide from near the amino-terminus of human AES.
Target Name
AES
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/mL.)

Western Blot (WB) (Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/mL.)

Immunocytochemistry (ICC) (Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/mL.)
Related Product Information for anti-AES antibody
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Product Categories/Family for anti-AES antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
166
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,091 Da
NCBI Official Full Name
amino-terminal enhancer of split isoform a
NCBI Official Synonym Full Names
amino-terminal enhancer of split
NCBI Official Symbol
AES
NCBI Official Synonym Symbols
GRG; ESP1; GRG5; TLE5; AES-1; AES-2; Grg-5
NCBI Protein Information
amino-terminal enhancer of split
UniProt Protein Name
Amino-terminal enhancer of split
Protein Family
UniProt Gene Name
AES
UniProt Synonym Gene Names
GRG; GRG5; Amino enhancer of split
UniProt Entry Name
AES_HUMAN

NCBI Description

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AES: Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Belongs to the WD repeat Groucho/TLE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus

Molecular Function: protein binding; transcription corepressor activity

Biological Process: cellular response to extracellular stimulus; multicellular organismal development; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; organ morphogenesis; regulation of growth; skeletal development; transcription, DNA-dependent; Wnt receptor signaling pathway

Research Articles on AES

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Product Notes

The AES aes (Catalog #AAA9412814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AES Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AES can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the AES aes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AES, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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