Sequence
Bone Morphogenetic Protein 2 (BMP2) Active Protein | BMP2 active protein
Active Bone Morphogenetic Protein 2 (BMP2)
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Activity
(Bone morphogenetic protein 2 (BMP2) belongs to the TGF-Beta superfamily of proteins. It plays an important role in the development of bone and cartilage. BMP2 is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It also paticipated in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. Besides, Follistatin Like Protein 1 (FSTL1) has been identified as an interactor of BMP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human BMP2 and recombinant human FSTL1. Briefly, BMP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100@L were then transferred to FSTL1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-BMP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times.With the addition of substrate solution , wells were incubated 15-25 minutes at 37 degree C. Finally, add 50@L stop solution to the wells and read at 450nm immediately. The binding activity of BMP2 and FSTL1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity
(Bone morphogenetic protein 2 (BMP2) belongs to the TGF-Beta superfamily of proteins. It plays an important role in the development of bone and cartilage. BMP2 is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It also paticipated in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. Besides, Follistatin Like Protein 1 (FSTL1) has been identified as an interactor of BMP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human BMP2 and recombinant human FSTL1. Briefly, BMP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100@L were then transferred to FSTL1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-BMP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times.With the addition of substrate solution , wells were incubated 15-25 minutes at 37 degree C. Finally, add 50@L stop solution to the wells and read at 450nm immediately. The binding activity of BMP2 and FSTL1 was shown in Figure 1, and this effect was in a dose dependent manner.)
SDS-PAGE
(Sample: Active recombinant BMP2, Human)
SDS-PAGE
(Sample: Active recombinant BMP2, Human)
Western Blot
(Western BlotSample: Recombinant BMP2, Human;Antibody: Rabbit Anti-Human BMP2 Ab (MBS2026337))
Western Blot
(Western BlotSample: Recombinant BMP2, Human;Antibody: Rabbit Anti-Human BMP2 Ab (MBS2026337))
NCBI and Uniprot Product Information
Accurate Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions.
NCBI Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]
Uniprot Description
Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).
Research Articles on BMP2
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Product Notes
The BMP2 bmp2 (Catalog #AAA2105049) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bone Morphogenetic Protein 2 (BMP2) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. Researchers should empirically determine the suitability of the BMP2 bmp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein 2 (BMP2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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