Western Blot (WB)
(Western blot analysis of NOB1 Antibody (C-term) in Y79 cell line lysates (35ug/lane). NOB1 (arrow) was detected using the purified Pab.)
Rabbit NOB1 Polyclonal Antibody | anti-NOB1 antibody
NOB1 Antibody (C-term)
Gene Names
NOB1; ART-4; NOB1P; MST158; MSTP158; PSMD8BP1
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NOB1; Polyclonal Antibody; NOB1 Antibody (C-term); RNA-binding protein NOB1; Phosphorylation regulatory protein HP-10; Protein ART-4; ART4; NOB1P; PSMD8BP1; anti-NOB1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NOB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-412 amino acids from the C-terminal region of human NOB1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
383-412
Sequence Length
412
Applicable Applications for anti-NOB1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of NOB1 Antibody (C-term) in Y79 cell line lysates (35ug/lane). NOB1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of NOB1 Antibody (C-term) in Y79 cell line lysates (35ug/lane). NOB1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-NOB1 antibody
NOB1 may play a role in mRNA degradation.
References
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Zhang, Y., et al. Mol. Biol. Rep. 32(3):185-189(2005)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Zhang, Y., et al. Mol. Biol. Rep. 32(3):185-189(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46675
NCBI Official Full Name
RNA-binding protein NOB1
NCBI Official Synonym Full Names
NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
NCBI Official Symbol
NOB1
NCBI Official Synonym Symbols
ART-4; NOB1P; MST158; MSTP158; PSMD8BP1
NCBI Protein Information
RNA-binding protein NOB1
UniProt Protein Name
RNA-binding protein NOB1
Protein Family
UniProt Gene Name
NOB1
UniProt Synonym Gene Names
ART4; NOB1P; PSMD8BP1
UniProt Entry Name
NOB1_HUMAN
NCBI Description
In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM, Nov 2010]
Uniprot Description
NOB1P: May play a role in mRNA degradation. Belongs to the NOB1 family.
Protein type: RNA processing
Chromosomal Location of Human Ortholog: 16q22.3
Cellular Component: nucleus
Molecular Function: metal ion binding
Biological Process: visual perception
Research Articles on NOB1
Similar Products
Product Notes
The NOB1 nob1 (Catalog #AAA9207732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 383-412. The NOB1 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NOB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the NOB1 nob1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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