Rabbit anti-Human, Mouse AES Polyclonal Antibody | anti-AES antibody

AES Antibody

Cat. Num. AAA150869

• Gene Names: AES; GRG; ESP1; GRG5; TLE5; AES-1; AES-2; Grg-5
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: AES Antibody is affinity chromatography purified via peptide column.
• Synonyms: AES; Polyclonal Antibody; AES Antibody; GRG; ESP1; GRG5; TLE5; AES-1; AES-2; Amino-terminal enhancer of split; Gp130-associated protein GAM; Amino enhancer of split; amino-terminal enhancer of split; anti-AES antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: AES Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 264

• Applicable Applications for anti-AES antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: AES antibody can be used for the detection of AES by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: AES antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human AES.
• Buffer: AES Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: AES antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of AES in 293 cells with AES antibody at 10 μg/mL. )

Immunofluorescence (IF)

(Immunofluorescence of AES in 293 cells with AES antibody at 20 μg/mL.)

Related Product Information for anti-AES antibody

AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

NCBI and Uniprot Product Information

• NCBI GI #: 39812019
• NCBI GeneID: 166
• NCBI Accession #: NP_945320
• NCBI GenBank Nucleotide #: NM_198969.1
• UniProt Accession #: Q08117; Q12808; Q14CJ1; Q96TG9; Q9UDY9; B2RBL0
• Molecular Weight: 29,091 Da
• NCBI Official Full Name: amino-terminal enhancer of split isoform a
• NCBI Official Synonym Full Names: amino-terminal enhancer of split
• NCBI Official Symbol: AES
• NCBI Official Synonym Symbols: GRG; ESP1; GRG5; TLE5; AES-1; AES-2; Grg-5
• NCBI Protein Information: amino-terminal enhancer of split; groucho-related protein 5; gp130-associated protein GAM
• UniProt Protein Name: Amino-terminal enhancer of split
• Protein Family: Acetyl esterase
• UniProt Gene Name: AES
• UniProt Synonym Gene Names: GRG; GRG5; Amino enhancer of split
• UniProt Entry Name: AES_HUMAN

NCBI Description

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AES: Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Belongs to the WD repeat Groucho/TLE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus

Molecular Function: protein binding; transcription corepressor activity

Biological Process: organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; multicellular organismal development; regulation of growth; cellular response to extracellular stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of protein binding; skeletal development