Western Blot (WB)
(Western blot analysis of Adrenergic Receptor alpha-1B using COS7 whole cell lysates)
Rabbit Adrenergic Receptor alpha-1B Polyclonal Antibody | anti-ADRA1B antibody
Adrenergic Receptor alpha-1B Antibody
Gene Names
ADRA1B; ADRA1; ALPHA1BAR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Adrenergic Receptor alpha-1B; Polyclonal Antibody; Adrenergic Receptor alpha-1B Antibody; ADA1B_HUMAN; ADRA 1; Adra 1b; ADRA1; ADRA1B; Adrenergic alpha 1B receptor; Adrenergic receptor alpha 1b; Adrenoceptor alpha 1B; Alpha 1 adrenergic receptor; Alpha 1B adrenoceptor; Alpha 1B adrenoreceptor; Alpha-1B adrenergic receptor; Alpha-1B adrenoceptor; Alpha-1B adrenoreceptor; Alpha1 adrenergic receptor; Alpha1B adrenergic receptor; Alpha1BAR; anti-ADRA1B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Adrenergic Receptor alpha-1B antibody detects endogenous levels of total Adrenergic Receptor alpha-1B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
520
Applicable Applications for anti-ADRA1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human Adrenergic Receptor alpha-1B
Subcellular Location
Nucleus Membrane. Cell Membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
Predicted Cross Reactivity
Rabbit, Dog, Chicken
Similarity
Rabbit (100%), Dog (92%), Chicken (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Adrenergic Receptor alpha-1B using COS7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of Adrenergic Receptor alpha-1B using COS7 whole cell lysates)
Related Product Information for anti-ADRA1B antibody
Function: This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Subunit Structure: Homo-and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes (PubMed:22120526). Interacts with CAVIN4 (PubMed:24567387).
Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
Subunit Structure: Homo-and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes (PubMed:22120526). Interacts with CAVIN4 (PubMed:24567387).
Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 56 kDa
Predicted: 57 kDa
Predicted: 57 kDa
NCBI Official Full Name
alpha-1B adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor alpha 1B
NCBI Official Symbol
ADRA1B
NCBI Official Synonym Symbols
ADRA1; ALPHA1BAR
NCBI Protein Information
alpha-1B adrenergic receptor
UniProt Protein Name
Alpha-1B adrenergic receptor
Protein Family
UniProt Gene Name
ADRA1B
UniProt Synonym Gene Names
Alpha-1B adrenoceptor
NCBI Description
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. [provided by RefSeq, Jul 2008]
Uniprot Description
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Research Articles on ADRA1B
Similar Products
Product Notes
The ADRA1B adra1b (Catalog #AAA9609115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Adrenergic Receptor alpha-1B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Adrenergic Receptor alpha-1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the ADRA1B adra1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adrenergic Receptor alpha-1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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