Typical Testing Data/Standard Curve
Mouse Alpha-1B adrenergic receptor (ADRA1B) ELISA Kit | ADRA1B elisa kit
Mouse Alpha-1B adrenergic receptor (ADRA1B) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||ADRA1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ADRA1B antibody and an ADRA1B-HRP conjugate. The assay sample and buffer are incubated together with ADRA1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRA1B concentration since ADRA1B from samples and ADRA1B-HRP conjugate compete for the anti-ADRA1B antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRA1B from the sample, fewer sites are left to bind ADRA1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRA1B concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. [provided by RefSeq, Jul 2008]
Uniprot Description
ADRA1B: a G protein-coupled catecholamine receptor that activates mitogenic responses and regulates growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. Can induce neoplastic transformation when transfected into cell lines. Can regulate the phosphorylation status of the STAT3 pathway. Mediates blood pressure and aorta contractile responses induced by alpha-1 agonists. Activates a phosphatidylinositol second messenger system.
Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q33.3
Cellular Component: nuclear membrane; integral to plasma membrane; plasma membrane; nucleus
Molecular Function: protein heterodimerization activity; alpha1-adrenergic receptor activity
Biological Process: adult heart development; vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure; positive regulation of the force of heart contraction by epinephrine-norepinephrine; multicellular organismal development; response to amphetamine; response to morphine; locomotory behavior; glucose homeostasis; positive regulation of glycogen catabolic process; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; organ growth; cell proliferation; positive regulation of MAPKKK cascade; cell-cell signaling; behavioral response to cocaine; regulation of vasoconstriction; visual learning; blood vessel remodeling; cell growth; positive regulation of heart rate by epinephrine-norepinephrine; negative regulation of glycogen catabolic process
Research Articles on ADRA1B
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Product Notes
The Mouse ADRA1B adra1b (Catalog #AAA7233695) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7233695 ELISA Kit recognizes Mouse ADRA1B. It is sometimes possible for the material contained within the vial of "Alpha-1B adrenergic receptor (ADRA1B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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