Rabbit ADPGK Polyclonal Antibody | anti-ADPGK antibody

ADPGK polyclonal antibody

Cat. Num. AAA3000173

• Gene Names: ADPGK; ADP-GK; 2610017G09Rik
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ADPGK; Polyclonal Antibody; ADPGK polyclonal antibody; ADP-dependent glucokinase; ADP-GK; RbBP-35; anti-ADPGK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ADPGK polyclonal antibody detects endogenous levels of ADPGK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Applicable Applications for anti-ADPGK antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human ADPGK.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of ADPGK polyclonal antibody at 1:500 dilutionLane1:The spleen tube tissue lysate of rat(40ug)Lane2:Raw264.7 whole cell lysate(40ug)Lane3:Hela whole cell lysate(40ug)Lane4:The spleen tube tissue lysate of mouse(40ug)Lane5:A549 whole cell lysate(40ug)Lane6:SHSY5Y whole cell lysate(40ug))

Related Product Information for anti-ADPGK antibody

Background: ADPGK (ADP-dependent glucokinase), also known as ADP-GK or RbBP-35, is a member of the ADP-dependent glucokinase family of proteins that areinvolved in both carbohydrate degradation and glycolysis. Expressed in a wide variety of tissues including lymphatic, endocrine, muscular and epithelial, ADPGK functions to catalyze the ADP-dependent phosphorylation of D-glucose to D-glucose 6-phosphate. Although GDP and CDP can replace ADP as a phosphate donor, the enzymatic efficiency of ADPGK is decreased when anything other than ADP is used. ADPGK contains one ADPK (ADPdependent kinase) domain and is able to bind one magnesium ion as a cofactor. Five isoforms of ADPGK exist due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 31542509
• NCBI GeneID: 83440
• NCBI Accession #: NP_112574.3
• NCBI GenBank Nucleotide #: NM_031284.4
• UniProt Accession #: Q9BRR6; Q49AU7; Q8NBI1; Q8WZ90; Q96NF8; Q9H0A7
• Molecular Weight: 54,089 Da
• NCBI Official Full Name: ADP-dependent glucokinase
• NCBI Official Synonym Full Names: ADP-dependent glucokinase
• NCBI Official Symbol: ADPGK
• NCBI Official Synonym Symbols: ADP-GK; 2610017G09Rik
• NCBI Protein Information: ADP-dependent glucokinase; rbBP-35; ATP-dependent glucokinase
• UniProt Protein Name: ADP-dependent glucokinase
• Protein Family: ADP-dependent glucokinase
• UniProt Gene Name: ADPGK
• UniProt Synonym Gene Names: ADP-GK; ADPGK
• UniProt Entry Name: ADPGK_HUMAN

NCBI Description

ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008]

Uniprot Description

ADPGK: Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency. Belongs to the ADP-dependent glucokinase family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.147; Secreted; Kinase, other; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: membrane; extracellular region

Molecular Function: ADP-specific glucokinase activity; metal ion binding

Biological Process: glycolysis; phosphorylation