Typical Testing Data/Standard Curve (for reference only)
Rat GLI Family Zinc Finger Protein 1 (GLI1) ELISA Kit | GLI1 elisa kit
Rat GLI Family Zinc Finger Protein 1 (GLI1) ELISA Kit
Gene Names
GLI1; GLI; PAPA8
Reactivity
Rat
Synonyms
GLI Family Zinc Finger Protein 1 (GLI1); Rat GLI Family Zinc Finger Protein 1 (GLI1) ELISA Kit; Glioma-Associated Oncogene Family Zinc Finger 1; GLI1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of GLI1. No significant cross-reactivity or interference between GLI1 and analogues was observed.
Sequence Length
1106
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Human Tissue Homogenates, Cell Lysates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.112ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLI1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLI1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GLI1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GLI1 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GLI1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GLI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GLI1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GLI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for GLI1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
113,676 Da
NCBI Official Full Name
GLI1
NCBI Official Synonym Full Names
GLI family zinc finger 1
NCBI Official Symbol
GLI1
NCBI Official Synonym Symbols
GLI; PAPA8
NCBI Protein Information
zinc finger protein GLI1
UniProt Protein Name
Zinc finger protein GLI1
Protein Family
UniProt Gene Name
GLI1
UniProt Synonym Gene Names
GLI
NCBI Description
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). May regulate the transcription of specific genes during normal development (PubMed:19706761). May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761). Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable).
Research Articles on GLI1
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Product Notes
The Rat GLI1 gli1 (Catalog #AAA2104973) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104973 ELISA Kit recognizes Rat GLI1. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the GLI1 gli1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLI Family Zinc Finger Protein 1 (GLI1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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