Rabbit anti-Human ADK Polyclonal Antibody | anti-adk antibody

ADK, CT (Adenosine Kinase, AK, Adenosine 5'-phosphotransferase)

Cat. Num. AAA6004698

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ADK; Polyclonal Antibody; CT (Adenosine Kinase; AK; Adenosine 5'-phosphotransferase); Anti -ADK; anti-adk antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADK.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-adk antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the C-terminal region of human ADK (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using 033720. This demonstrates that 033720 detected the CDR2 protein (arrow).)

Immunohistochemistry (IHC)

(Iimmunohistochemistry analysis of formalin fixed and paraffin embedded human cerebellum tissue using 033720 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 033720 for immunohistochemistry.)

Related Product Information for anti-adk antibody

Adenosine kinase is an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity.

Product Categories/Family for anti-adk antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 154350506
• NCBI GeneID: 5460952
• UniProt Accession #: A7Z0Q9
• Molecular Weight: 24,107 Da
• NCBI Official Full Name: Adk
• NCBI Official Symbol: adk
• NCBI Protein Information: adenylate kinase
• UniProt Protein Name: Adenylate kinase
• UniProt Gene Name: adk
• UniProt Synonym Gene Names: AK
• UniProt Entry Name: KAD_BACA2

Uniprot Description

Function: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism

By similarity. HAMAP-Rule MF_00235

Catalytic activity: ATP + AMP = 2 ADP. HAMAP-Rule MF_00235

Pathway: Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1. HAMAP-Rule MF_00235

Subunit structure: Monomer

By similarity. HAMAP-Rule MF_00235

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_00235.

Domain: Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain

By similarity. HAMAP-Rule MF_00235

Sequence similarities: Belongs to the adenylate kinase family.