Rabbit NR3C1 Polyclonal Antibody | anti-NR3C1 antibody

NR3C1 Polyclonal Antibody

Cat. Num. AAA3013150

• Gene Names: NR3C1; GR; GCR; GRL; GCCR; GCRST
• Reactivity: Human, Mouse, Rat, Zebrafish
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: NR3C1; Polyclonal Antibody; NR3C1 Polyclonal Antibody; GR; GCCR; GCR; GCRST; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; GRL; Grl1; nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3; group C; member 1 (glucocorticoid receptor); anti-NR3C1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NR3C1 polyclonal antibody detects endogenous levels of NR3C1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 777

• Applicable Applications for anti-NR3C1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000-1:5,000; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Glucocorticoid Receptor on different lysates using anti-Glucocorticoid Receptor antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: A549 Lane 3: HepG2)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NIH/3T3 cells with Glucocorticoid Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

Related Product Information for anti-NR3C1 antibody

The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR alpha. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.

NCBI and Uniprot Product Information

• NCBI GI #: 4504133
• NCBI GeneID: 2908
• NCBI Accession #: NP_000167.1
• NCBI GenBank Nucleotide #: NP_000167.1
• UniProt Accession #: P04150; P06537; P06536
• Molecular Weight: 86kDa
• NCBI Official Full Name: glucocorticoid receptor isoform alpha
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3 group C member 1
• NCBI Official Symbol: NR3C1
• NCBI Official Synonym Symbols: GR; GCR; GRL; GCCR; GCRST
• NCBI Protein Information: glucocorticoid receptor
• UniProt Protein Name: Glucocorticoid receptor
• Protein Family: Glucocorticoid receptor
• UniProt Gene Name: NR3C1
• UniProt Synonym Gene Names: GRL; GR
• UniProt Entry Name: GCR_HUMAN

NCBI Description

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Uniprot Description

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding; Mitochondrial; Nuclear receptor

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; glucocorticoid receptor activity; protein binding; zinc ion binding; transcription factor activity; steroid binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; maternal behavior; adrenal gland development; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid metabolic process; glucocorticoid mediated signaling; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of gluconeogenesis

Disease: Glucocorticoid Resistance, Generalized