Rabbit anti-Human, Mouse ADK Polyclonal Antibody | anti-adk antibody

ADK, NT (Adenosine Kinase, AK, Adenosine 5'-phosphotransferase)

Cat. Num. AAA6001207

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ADK; Polyclonal Antibody; NT (Adenosine Kinase; AK; Adenosine 5'-phosphotransferase); Anti -ADK; anti-adk antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADK. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-adk antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human ADK (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot to detect ADK in mouse bladder tissue lysate using MBS6001207.)

Related Product Information for anti-adk antibody

Adenosine kinase (ATP:adenosine 5-prime-phosphotransferase) is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a potentially important regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of ADK could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as antiinflammatory agents. The encoded protein does not present any sequence similarities to other well-characterized mammalian nucleoside kinases. In contrast, 2 regions were identified with significant sequence identity to microbial ribokinase and fructokinases and a bacterial inosine/guanosine kinase. Thus, ADK is a structurally distinct mammalian nucleoside kinase that appears to be akin to sugar kinases of microbial origin. Animal studies have demonstrated that a deficiency of adenosine metabolism a powerful contributor to the development of neonatal hepatic steatosis, providing a model for the rapid development of postnatally lethal fatty liver.

Product Categories/Family for anti-adk antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 154350506
• NCBI GeneID: 5460952
• UniProt Accession #: A7Z0Q9
• Molecular Weight: 24,107 Da
• NCBI Official Full Name: Adk
• NCBI Official Symbol: adk
• NCBI Protein Information: adenylate kinase
• UniProt Protein Name: Adenylate kinase
• UniProt Gene Name: adk
• UniProt Synonym Gene Names: AK
• UniProt Entry Name: KAD_BACA2

Uniprot Description

Function: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism

By similarity. HAMAP-Rule MF_00235

Catalytic activity: ATP + AMP = 2 ADP. HAMAP-Rule MF_00235

Pathway: Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1. HAMAP-Rule MF_00235

Subunit structure: Monomer

By similarity. HAMAP-Rule MF_00235

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_00235.

Domain: Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain

By similarity. HAMAP-Rule MF_00235

Sequence similarities: Belongs to the adenylate kinase family.