ADK, aa22- 362 Recombinant Protein | adk recombinant protein

ADK, aa22- 362, Recombinant, Human (Adenosine 5'-phosphotransferase, Adenosine Kinase, AK)

Cat. Num. AAA635687

• Purity: Purified; ~95% (SDS-PAGE)
• Synonyms: ADK; aa22- 362; Recombinant; Human (Adenosine 5'-phosphotransferase; Adenosine Kinase; AK); adk recombinant protein

• Host: E Coli
• Purity/Purification: Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 20% glycerol, 1mM EDTA, 50mM sodium chloride.

• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(15% SDS-PAGE using 3ug of A0882-62.)

Related Product Information for adk recombinant protein

Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents.

Recombinant protein corresponding to aa22-362 from human ADK fused to a 6-His tag at the N-terminal expressed in E. coli (NP_006712).

Product Categories/Family for adk recombinant protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 154350506
• NCBI GeneID: 5460952
• UniProt Accession #: A7Z0Q9
• Molecular Weight: 40.5kD
• NCBI Official Full Name: Adk
• NCBI Official Symbol: adk
• NCBI Protein Information: adenylate kinase
• UniProt Protein Name: Adenylate kinase
• UniProt Gene Name: adk
• UniProt Synonym Gene Names: AK
• UniProt Entry Name: KAD_BACA2

Uniprot Description

Function: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism

By similarity. HAMAP-Rule MF_00235

Catalytic activity: ATP + AMP = 2 ADP. HAMAP-Rule MF_00235

Pathway: Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1. HAMAP-Rule MF_00235

Subunit structure: Monomer

By similarity. HAMAP-Rule MF_00235

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_00235.

Domain: Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain

By similarity. HAMAP-Rule MF_00235

Sequence similarities: Belongs to the adenylate kinase family.