Human PROC (Protein C) CLIA Kit | PROC clia kit

Human PROC (Protein C) CLIA Kit

Cat. Num. AAA2532389

• Gene Names: PROC; PC; APC; PROC1; THPH3; THPH4
• Reactivity: Human
• Synonyms: PROC (Protein C); Human PROC (Protein C) CLIA Kit; PROC clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant PROC. No significant cross-reactivity or interference between PROC and analogues was observed.
• Sequence Length: 84

• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PROC clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of PROC concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PROC. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PROC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PROC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PROC. You can calculate the concentration of PROC in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 265668
• NCBI GeneID: 5624
• Molecular Weight: 57,556 Da
• NCBI Official Full Name: protein C, partial
• NCBI Official Synonym Full Names: protein C (inactivator of coagulation factors Va and VIIIa)
• NCBI Official Symbol: PROC
• NCBI Official Synonym Symbols: PC; APC; PROC1; THPH3; THPH4
• NCBI Protein Information: vitamin K-dependent protein C
• UniProt Protein Name: Vitamin K-dependent protein C
• Protein Family: Vitamin K-dependent protein
• UniProt Gene Name: PROC
• UniProt Entry Name: PROC_HUMAN

NCBI Description

This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]

Uniprot Description

PROC: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal dominant (THPH3). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Individuals with decreased amounts of protein C are classically referred to as having type I protein C deficiency and those with normal amounts of a functionally defective protein as having type II deficiency. Defects in PROC are the cause of thrombophilia due to protein C deficiency, autosomal recessive (THPH4). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. It results in a thrombotic condition that can manifest as a severe neonatal disorder or as a milder disorder with late-onset thrombophilia. The severe form leads to neonatal death through massive neonatal venous thrombosis. Often associated with ecchymotic skin lesions which can turn necrotic called purpura fulminans, this disorder is very rare. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.69; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 2q13-q14

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity; calcium ion binding

Biological Process: cellular protein metabolic process; negative regulation of blood coagulation; post-translational protein modification; blood coagulation; proteolysis; leukocyte migration; peptidyl-glutamic acid carboxylation; negative regulation of apoptosis

Disease: Thrombophilia Due To Protein C Deficiency, Autosomal Recessive; Thrombophilia Due To Protein C Deficiency, Autosomal Dominant