Rabbit Adenosine A1 Receptor Polyclonal Antibody | anti-ADORA1 antibody

Adenosine A1 Receptor

Cat. Num. AAA616447

• Gene Names: ADORA1; RDC7
• Reactivity: Guinea Pig, Human, Porcine, Rat, Sheep
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Adenosine A1 Receptor; Polyclonal Antibody; Anti -Adenosine A1 Receptor; anti-ADORA1 antibody

• Host: Rabbit
• Reactivity: Guinea Pig, Human, Porcine, Rat, Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Adenosine A1 receptor (A1R) at 79 and 39kD bands in cortical membranes. The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent A1R dimers. Species Crossreactivity: human, porcine, sheep and guinea pig.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA.

• Applicable Applications for anti-ADORA1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.; Dilution: ELISA: 1:10,000-1:50,000; Western Blot (ECL): 1-10ug/ml. Use chemiluminescence technique. Higher concentrations of antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.; Immunohistochemistry (Frozen): 10-50ug/ml Light fixation with 0.2% picric acid is recommended.; Immunocytochemistry: Light 2% PFA fixation for 5-10 minutes is recommended.
• Immunogen: Synthetic peptide corresponding to the 3rd extracellular domain aa163-176, GEPVIKAEFEKVIS, of the rat A1R gene.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ADORA1 antibody

The A1 Adenosine Receptor is involved in smooth muscle contraction, control of heart rate, sedation, and lipolysis. Expression of the adenosine A1 receptor has been reported in the central nervous system, particularly in the cerebral cortex, hippocampus, thalamus, striatum, and cerebellum, and in peripheral tissues including heart, adipose, testis, and kidney. ESTs have been isolated from brain, breast, eye, kidney, salivary gland, and testis libraries.

Product Categories/Family for anti-ADORA1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 54696570
• NCBI GeneID: 134
• UniProt Accession #: P30542; Q6FHK3; Q8TAM8; A6NFY5; A6NGP4; A8K1L3; B3KXQ4
• Molecular Weight: 36,512 Da
• NCBI Official Full Name: adenosine A1 receptor
• NCBI Official Synonym Full Names: adenosine A1 receptor
• NCBI Official Symbol: ADORA1
• NCBI Official Synonym Symbols: RDC7
• NCBI Protein Information: adenosine receptor A1; OTTHUMP00000039090; OTTHUMP00000039091
• UniProt Protein Name: Adenosine receptor A1
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA1
• UniProt Entry Name: AA1R_HUMAN

NCBI Description

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq]

Uniprot Description

ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Endoplasmic reticulum; Membrane protein, multi-pass; Apoptosis; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: endoplasmic reticulum; integral to plasma membrane; basolateral plasma membrane; postsynaptic density; dendritic spine; terminal button; axolemma; asymmetric synapse; presynaptic membrane; postsynaptic membrane; cell soma; plasma membrane; presynaptic active zone

Molecular Function: protein binding; G-protein-coupled receptor binding; protein heterodimerization activity; G-protein beta/gamma-subunit binding; adenosine receptor activity, G-protein coupled; purine nucleoside binding; phospholipase C activity; heterotrimeric G-protein binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; relaxation of vascular smooth muscle; negative regulation of glutamate secretion; activation of MAPKK activity; negative regulation of hormone secretion; positive regulation of potassium ion transport; regulation of synaptic plasticity; positive regulation of peptide secretion; neurological control of breathing; thermoregulation; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of glomerular filtration; positive regulation of epidermal growth factor receptor activity; cell-cell signaling; positive regulation of nucleoside transport; negative regulation of vasodilation; negative regulation of lipid catabolic process; negative regulation of blood pressure; detection of temperature stimulus involved in sensory perception of pain; protein targeting to membrane; negative regulation of synaptic transmission, GABAergic; inflammatory response; adenosine receptor signaling pathway; nervous system development; negative regulation of neurotrophin production; negative regulation of acute inflammatory response; negative regulation of synaptic transmission, glutamatergic; negative regulation of leukocyte migration; phagocytosis; response to hypoxia; regulation of sensory perception of pain; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of excitatory postsynaptic membrane potential; cognition; positive regulation of blood pressure; lipid catabolic process; negative regulation of apoptosis