Goat HDAC6 Polyclonal Antibody | anti-HDAC6 antibody

Goat Polyclonal to Human HDAC6

Cat. Num. AAA248082

• Gene Names: HDAC6; HD6; JM21; CPBHM; PPP1R90
• Reactivity: Gibbon, Chimpanzee, Human, Monkey, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: HDAC6; Polyclonal Antibody; Goat Polyclonal to Human HDAC6; Anti-HDAC6 Antibody (C-Terminus) IHC-plus; Histone deacetylase 6; HD6; KIAA0901; JM21; Human HDAC6; anti-HDAC6 antibody

• Host: Goat
• Reactivity: Gibbon, Chimpanzee, Human, Monkey, Mouse
• Clonality: Polyclonal
• Specificity: Human HDAC6.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1215

• Applicable Applications for anti-HDAC6 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 150kD band observed in nuclear lysates of cell lines NIH3T3, and HeLa (calculated MW of 131kD according to NP_006035.2). Recommended concentration: 1-3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-AHQNKFGEDMPHPH from the C-terminus of human HDAC6 (NP_006035.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Mouse, Panda, Rabbit, Bat, Elephant (93%); Horse, Guinea pig (86%); Rat (79%).
• Immunogen Type: Synthetic peptide
• Immunogen: HDAC6 antibody was raised against synthetic peptide C-AHQNKFGEDMPHPH from the C-terminus of human HDAC6 (NP_006035.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Mouse, Panda, Rabbit, Bat, Elephant (93%); Horse, Guinea pig (86%); Rat (79%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-HDAC6 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(HDAC6 antibody (1 ug/ml) staining of HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-HDAC6 antibody

Family: Histone Deacetylase

NCBI and Uniprot Product Information

• NCBI GI #: 13128864
• NCBI GeneID: 10013
• NCBI Accession #: NP_006035.2
• NCBI GenBank Nucleotide #: NM_006044.2
• UniProt Accession #: Q9UBN7; O94975; Q6NT75; Q7L3E5; Q96CY0
• Molecular Weight: 114,361 Da
• NCBI Official Full Name: histone deacetylase 6
• NCBI Official Synonym Full Names: histone deacetylase 6
• NCBI Official Symbol: HDAC6
• NCBI Official Synonym Symbols: HD6; JM21; CPBHM; PPP1R90
• NCBI Protein Information: histone deacetylase 6; protein phosphatase 1, regulatory subunit 90
• UniProt Protein Name: Histone deacetylase 6
• UniProt Gene Name: HDAC6
• UniProt Synonym Gene Names: KIAA0901; HD6
• UniProt Entry Name: HDAC6_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily.

Protein type: Ubiquitin conjugating system; Deacetylase; EC 3.5.1.98; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: microtubule; dendrite; leading edge; histone deacetylase complex; perikaryon; caveola; inclusion body; cytosol; nucleoplasm; dynein complex; microtubule associated complex; cytoplasmic microtubule; axon; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: zinc ion binding; histone deacetylase binding; microtubule binding; beta-tubulin binding; beta-catenin binding; Hsp90 protein binding; misfolded protein binding; actin binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); tubulin deacetylase activity; ubiquitin protein ligase binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; polyubiquitin binding; tau protein binding; alpha-tubulin binding

Biological Process: negative regulation of proteolysis; response to misfolded protein; protein polyubiquitination; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; positive regulation of signal transduction; macroautophagy; negative regulation of microtubule depolymerization; regulation of fat cell differentiation; organelle organization and biogenesis; response to toxin; misfolded or incompletely synthesized protein catabolic process; histone deacetylation; regulation of gene expression, epigenetic; response to organic substance; intracellular protein transport; protein complex disassembly; lysosome localization; protein amino acid deacetylation; negative regulation of oxidoreductase activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent; negative regulation of protein complex disassembly

Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia