Rat T lymphocyte subpopulation CD3/4/8 ELISA Kit | TLS elisa kit

Rat T lymphocyte subpopulation CD3/4/8 ELISA Kit

Cat. Num. AAA729871

• Gene Names: FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• Reactivity: Rat
• Synonyms: T lymphocyte subpopulation CD3/4/8; Rat T lymphocyte subpopulation CD3/4/8 ELISA Kit; TLS elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/ml
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLS elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This TLS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TLS. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY TLS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLS antibody and an TLS-HRP conjugate. The assay sample and buffer are incubated together with TLS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLS concentration since TLS from samples and TLS-HRP conjugate compete for the anti-TLS antibody binding site. Since the number of sites is limited, as more sites are occupied by TLS from the sample, fewer sites are left to bind TLS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLS concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TLS elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 386157
• NCBI GeneID: 2521
• Molecular Weight: 53,355 Da
• NCBI Official Full Name: TLS
• NCBI Official Synonym Full Names: FUS RNA binding protein
• NCBI Official Symbol: FUS
• NCBI Official Synonym Symbols: TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• NCBI Protein Information: RNA-binding protein FUS; oncogene FUS; oncogene TLS; fus-like protein; fused in sarcoma; 75 kDa DNA-pairing protein; fusion gene in myxoid liposarcoma; translocated in liposarcoma protein; heterogeneous nuclear ribonucleoprotein P2
• UniProt Protein Name: RNA-binding protein FUS
• UniProt Gene Name: FUS
• UniProt Synonym Gene Names: TLS
• UniProt Entry Name: FUS_HUMAN

NCBI Description

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]

Uniprot Description

hnRNP P2: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single- stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. Ubiquitous. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; RNA splicing; RNA-binding; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression

Disease: Amyotrophic Lateral Sclerosis 6, With Or Without Frontotemporal Dementia; Tremor, Hereditary Essential, 4