Rabbit anti-Human ADAM-9 (A Disintigrin And Metalloproteinase-8, MDC9, Meltrin-g) Polyclonal Antibody | anti-ADAM-9 antibody

Anti-ADAM-9 (A Disintigrin And Metalloproteinase-8, MDC9, Meltrin-g), Propeptide Domain Antibody

Cat. Num. AAA4157448

• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: ADAM-9 (A Disintigrin And Metalloproteinase-8; MDC9; Meltrin-g); Polyclonal Antibody; Anti-ADAM-9 (A Disintigrin And Metalloproteinase-8; Propeptide Domain Antibody; anti-ADAM-9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for ADAM9, also known as MDC9 and Meltrin-g.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-ADAM-9 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Detects bands of approximately 84kDa and 114kDa, due to glycosylation and cyteine-rich regions (predicted molecular weight: 91 kDa). Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the amino end (propeptide)of human ADAM9.
• Cellular Localization: Isoform 1: Cell Membrane; Single-Pass Type I Membrane Protein. Isoform 2: Secreted protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-ADAM-9 antibody

Description: The antibody is a polyclonal antibody made to the metalloproteinase ADAM-9. The antibody is made to a synthetic peptide based on the amino end (propeptide)of human ADAM-9. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. ADAM-9, also known as MDC9 and Meltrin-g, was first described in muscle cells as a protein with homology to the fertilins (ADAMs 1, 2). Initial observations indicated a role for ADAM9 in myoblast fusion, similar to sperm-egg fusion aided by ADAMs 1 and 2. Later works describe ADAM9 in the lung, heart, brain, kidney, and a wide range of tissues, and examined the ability of ADAM9 to activate TNF-a and HB-EGF, and act as an adhesion molecule with integrin avb5. The Cytoplasmic domain of ADAM9 contains SH3 ligand domains, which are thought to interact with PKC-d, suggesting specific regulation routes for ADAM9. A novel form of ADAM9 was reported in myeloma cells, with truncated amino and carboxyterminal ends, and a 13 amino acid insert in the cysteine-rich domain. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the function of ADAM-9 is still poorly understood. ADAM-9 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM9 sequence codes for an 819 amino acid protein, containing a Type-I transmembrane domain. Predicted mass is 90.5 kD, but glycosylation and cyteine-rich regions make the protein run at 114 kD unprocessed, and 84 kD processed forms. Mouse ADAM9 sequence is 845 AA, predicted at 92 kD. RP1ADAM-9 recognizes bands of 116 kD (minor band), 84 kD (major band), and breakdown products recover at 50 kD, 34 kD in reduced Western blots of cell lysates. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

Relevance: ADAM9 encodes disintegrin and metalloprotease (ADAM) domain 9, which is a member of the ADAM protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The member encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor.