Rabbit anti-Human ADAM-15 (A Disintigrin And Metalloproteinase-15, Metargidin, MDC15) Polyclonal Antibody | anti-ADAM-15 antibody

Anti-ADAM-15 (A Disintigrin And Metalloproteinase-15, Metargidin, MDC15), Cytoplasmin Domain Antibody

Cat. Num. AAA4157460

• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: ADAM-15 (A Disintigrin And Metalloproteinase-15; Metargidin; MDC15); Polyclonal Antibody; Anti-ADAM-15 (A Disintigrin And Metalloproteinase-15; Cytoplasmin Domain Antibody; anti-ADAM-15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody recognises the cytoplasmic domain of ADAM15. This antibody recognizes zymogen and furin processed forms of ADAM15.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-ADAM-15 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 (when using colorimetric substrates such as BCIP/NBT) and 1:5000 (for chemiluminescent substrates). Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa). Note: The antibody recognizes bands of 110 kDa and 90 kDa, the zymogen and furin processed forms of ADAM15. A series of cleaved products_recover at 55 kDa and 40 kDa can also be seen in reduced Western blots of cell lysates. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the cytoplasmic domain of human ADAM15.
• Cellular Localization: Membrane; Single Pass Type I Membrane Protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-ADAM-15 antibody

Description: The antibody is a polyclonal antibody made to the metalloproteinase ADAM-15. The antibody is made to a synthetic peptide based on the Cytoplasmic domain of human ADAM-15. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. ADAM-15, also known as Metargidin and MDC15, was first described in mammary epithelial carcinoma cells as a membrane-anchored Metalloprotease-Disintegrin with RGD sequence (MetaRGiDin). Mouse ADAM15 does not contain the RGD sequence: instead the mouse ADAM15 has a TDD in its place. Like ADAM9 and ADAM12, there are SH3 ligand domains in the Cytoplasmic portion of ADAM15, suggesting regulation routes for ADAM15 via Src and Src tyrosine kinase. ADAM15 is widely expressed in normal tissues and tumor cell lines, and is expressed on the cell surface, anchored by the transmembrane domain. ADAM15 is also elevated in osteoarthric and rheumatoid synovial tissues, suggesting an inflammatory role. ADAM15 may also act as a cell-attachment molecule, by binding the integrins avb3, a9b1 and avb5 through the cysteine-rich domain and/or RGD sequence. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the functions of ADAM-15 are still poorly understood. ADAM-15 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM15 sequence codes for a 814 amino acid protein. Predicted mass is 85 kD, but glycosylation and cyteine-rich regions make the reduced protein run at 110 kD unprocessed, and 84 kD processed forms. RP1ADAM-15 recognizes bands of 110 kDand 90 kD, the zymogen and furin-processed forms of ADAM15. A series of cleaved products_recover at 55 kD, 40 kD can also be seen in reduced Western blots of cell lysates. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

Relevance: ADAM15, also known as Metargidin and MDC15, is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It was first described in mammary epithelial carcinoma cells as a membrane anchored Metalloprotease Disintegrin with RGD sequence (MetaRGiDin). Mouse ADAM15 does not contain the RGD sequence; instead the mouse ADAM15 has a TDD in its place. Like ADAM9 and ADAM12, there are SH3 ligand domains in the cytoplasmic portion of ADAM15, suggesting regulation routes for ADAM15 via Src and Src tyrosine kinase. ADAM15 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Full length ADAM15, 814 amino acids, has a predicted mass of 85 kDa, but due to glycosylation and cysteine rich regions, the reduced protein migrates to 110 kDa (unprocessed) and 84 kDa (processed). ADAM15 is widely expressed in normal tissues and tumor cell lines, and is expressed on the cell surface, anchored by the transmembrane domain. It is also elevated in osteoarthritic and rheumatoid synovial tissues, suggesting an inflammatory role. ADAM15 may also act as a cell attachment molecule by binding the integrins avbeta3 and a5beta1 through the cysteine rich domain and/or RGD sequence.