Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 (PLCG2) Standard Curve.)
Mouse 1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase gamma-2 (PLCG2) ELISA Kit | PLCG2 elisa kit
Mouse 1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase gamma-2 (PLCG2) ELISA Kit
Gene Names
Plcg2; Plcg-2; PLCgamma2; PLC-gamma-2
Reactivity
Mouse
Synonyms
1-Phosphatidylinositol-4; 5-bisphosphate Phosphodiesterase gamma-2 (PLCG2); Mouse 1-Phosphatidylinositol-4; 5-bisphosphate Phosphodiesterase gamma-2 (PLCG2) ELISA Kit; PLCG2; Phospholipase C gamma 2; phospholipase C; gamma 2; gamma 2 (phosphatidylyinositol-specific); PLCG2 elisa kit
Reactivity
Mouse
Specificity
Mouse 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 (PLCG2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLCG2. No significant cross-reactivity or interference between Mouse PLCG2 and analogues was observed.
Sequence Length
1265
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 (PLCG2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 (PLCG2) Standard Curve.)
Related Product Information for PLCG2 elisa kit
Description: This Mouse 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 (PLCG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PLCG2 in samples. An antibody specific for PLCG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLCG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLCG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLCG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Overexpressed wildtype rat Plcg1 or a lipase-inactive mutant Plcg1 each augmented ACE in rat PC12 cells, while a deletion mutant lacking the region containing the SH3 domain of Plcg1 was ineffective. RNA interference to deplete either Plcg1 or Plcg2 in PC12 and rat aortic smooth muscle A7r5 cells inhibited ACE. In chicken DT40 B lymphocytes expressing only Plcg2, overexpressed human muscarinic M5 receptors (M5R) activated ACE. Using DT40 PLC2 knockout cells, M5R stimulation of endoplasmic reticulum Ca(2+) store release was unaffected, but ACE was abolished. Normal ACE was restored by transient expression of rat Plcg2 or a lipase-inactive Plcg2 mutant. The results indicated a lipase-independent role of PLCG in the physiologic agonist-induced activation of Ca(2+) entry.
Background: Overexpressed wildtype rat Plcg1 or a lipase-inactive mutant Plcg1 each augmented ACE in rat PC12 cells, while a deletion mutant lacking the region containing the SH3 domain of Plcg1 was ineffective. RNA interference to deplete either Plcg1 or Plcg2 in PC12 and rat aortic smooth muscle A7r5 cells inhibited ACE. In chicken DT40 B lymphocytes expressing only Plcg2, overexpressed human muscarinic M5 receptors (M5R) activated ACE. Using DT40 PLC2 knockout cells, M5R stimulation of endoplasmic reticulum Ca(2+) store release was unaffected, but ACE was abolished. Normal ACE was restored by transient expression of rat Plcg2 or a lipase-inactive Plcg2 mutant. The results indicated a lipase-independent role of PLCG in the physiologic agonist-induced activation of Ca(2+) entry.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
NCBI Official Synonym Full Names
phospholipase C, gamma 2
NCBI Official Symbol
Plcg2
NCBI Official Synonym Symbols
Plcg-2; PLCgamma2; PLC-gamma-2
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Protein Family
UniProt Gene Name
Plcg2
UniProt Synonym Gene Names
PLC-gamma-2
UniProt Entry Name
PLCG2_MOUSE
Research Articles on PLCG2
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Product Notes
The Mouse PLCG2 plcg2 (Catalog #AAA9717339) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717339 ELISA Kit recognizes Mouse PLCG2. It is sometimes possible for the material contained within the vial of "1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase gamma-2 (PLCG2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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