Rabbit ADA Polyclonal Antibody | anti-ADA antibody

ADA Antibody

Cat. Num. AAA9413586

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: ADA; Polyclonal Antibody; ADA Antibody; ADA1; anti-ADA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total ADA protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 363

• Applicable Applications for anti-ADA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human ADA.
• Target Name: ADA
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ADA antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using ADA antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-ADA antibody

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.

Product Categories/Family for anti-ADA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 47078295
• NCBI GeneID: 100
• NCBI Accession #: NP_000013.2
• NCBI GenBank Nucleotide #: NM_000022.2
• UniProt Accession #: P00813; Q53F92; Q6LA59
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: adenosine deaminase
• NCBI Official Synonym Full Names: adenosine deaminase
• NCBI Official Symbol: ADA
• NCBI Protein Information: adenosine deaminase
• UniProt Protein Name: Adenosine deaminase
• Protein Family: ADAM
• UniProt Gene Name: ADA
• UniProt Synonym Gene Names: ADA1
• UniProt Entry Name: ADA_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.

Protein type: EC 3.5.4.4; Hydrolase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: dendrite cytoplasm; extracellular space; cell surface; cell soma; membrane; lysosome; cytoplasm; plasma membrane; cell junction; cytosol; external side of plasma membrane

Molecular Function: adenosine deaminase activity; protein binding; zinc ion binding; purine nucleoside binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; adenosine catabolic process; T cell activation; deoxyadenosine catabolic process; response to morphine; positive regulation of calcium-mediated signaling; purine salvage; positive regulation of T cell differentiation in the thymus; histamine secretion; purine ribonucleoside monophosphate biosynthetic process; response to vitamin E; regulation of cell-cell adhesion mediated by integrin; positive regulation of T cell receptor signaling pathway; negative regulation of mature B cell apoptosis; positive regulation of germinal center formation; positive regulation of B cell proliferation; hypoxanthine salvage; positive regulation of smooth muscle contraction; negative regulation of adenosine receptor signaling pathway; embryonic gut development; aging; placenta development; response to drug; Peyer's patch development; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of heart rate; liver development; negative regulation of leukocyte migration; purine base metabolic process; trophectodermal cell differentiation; purine nucleotide salvage; xanthine biosynthetic process; response to hydrogen peroxide; negative regulation of inflammatory response; response to hypoxia; inosine biosynthetic process; germinal center B cell differentiation; alveolus development; positive regulation of alpha-beta T cell differentiation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency