Rabbit anti-Human CRFR2 Polyclonal Antibody | anti-CRFR2 antibody

CRFR2 (D35) (CRHR2, CRF2R, CRH2R, Corticotropin-releasing factor receptor 2, Corticotropin-releasing hormone receptor 2) (Biotin)

Cat. Num. AAA6288782

• Gene Names: CRHR2; CRF2; CRFR2; CRF-RB; HM-CRF
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CRFR2; Polyclonal Antibody; CRFR2 (D35) (CRHR2; CRF2R; CRH2R; Corticotropin-releasing factor receptor 2; Corticotropin-releasing hormone receptor 2) (Biotin); CRHR2; Corticotropin-releasing hormone receptor 2; anti-CRFR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CRFR2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CRFR2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 10-39 of human CRFR2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using 034220. This demonstrates that 034220 detected the CRFR2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using 034220 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 034220 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using 034220 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CRFR2 antibody

Antibodies; Receptors

References

1. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 2. Gutknecht, E., et al. Eur. J. Pharmacol. 642 (1-3), 1-9 (2010). 3. Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). 4. Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010). 5. Joubert, B.R., et al. Genome Med 2 (3), 17 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 32307159
• NCBI GeneID: 1395
• NCBI Accession #: NP_001874.2
• NCBI GenBank Nucleotide #: NM_001883.4
• UniProt Accession #: Q13324; O43461; Q4QRJ4; Q99431; B2R967; B3SXS6; B3SXS7; B3SXS8; B3SXT0; F8WA81
• Molecular Weight: 47,560 Da
• NCBI Official Full Name: corticotropin-releasing factor receptor 2 isoform 1
• NCBI Official Synonym Full Names: corticotropin releasing hormone receptor 2
• NCBI Official Symbol: CRHR2
• NCBI Official Synonym Symbols: CRF2; CRFR2; CRF-RB; HM-CRF
• NCBI Protein Information: corticotropin-releasing factor receptor 2; CRH-R2; CRH receptor 2 variant B; CRF2 receptor, beta isoform
• UniProt Protein Name: Corticotropin-releasing factor receptor 2
• UniProt Gene Name: CRHR2
• UniProt Synonym Gene Names: CRF2R; CRH2R; CRF-R-2; CRF-R2; CRFR-2; CRH-R-2; CRH-R2
• UniProt Entry Name: CRFR2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jan 2011]

Uniprot Description

CRHR2: This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 2

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: Golgi apparatus; cell surface; integral to plasma membrane; endoplasmic reticulum; plasma membrane

Molecular Function: protein binding; hormone activity; corticotrophin-releasing factor receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of angiogenesis