Rabbit Fractalkine Polyclonal Antibody | anti-CX3CL1 antibody

Fractalkine Antibody

Cat. Num. AAA840223

• Gene Names: CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: Fractalkine; Polyclonal Antibody; Fractalkine Antibody; C-X3-C motif chemokine 1; Neurotactin; CX3C membrane-anchored chemokine; Small-inducible cytokine D1; anti-CX3CL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-Fractalkine polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 381

• Applicable Applications for anti-CX3CL1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western blot analysis (0.5-4 ug/ml). Per researcher's feedback, it can also be used in immunoprecipitation (4-8 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually.
• Immunogen: E. coli-expressed recombinant human Fractalkine
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-CX3CL1 antibody

Background: Fractalkine, also known as neurotactin, is a membrane-bound CX3C Chemokine. The mature protein is part of a 397-amino acid precursor and consists of a chemokine domain of 76 amino acids, a mucin stalk of 241 amino acids, a putative transmembrane domain of 18 amino acids, and an intracellular tail of 37 amino acids. Within the chemokine domain, the first two cysteine residues are separated by 3 amino acids (CX3C). Fractalkine message is found in high abundance in the brain, kidney, lung and heart tissues. Fractalkine is chemotactic for monocytes and other leukocytes including NK cells and may play a role in brain inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 62087654
• NCBI GeneID: 6376
• Molecular Weight: 42,203 Da
• NCBI Official Full Name: chemokine (C-X3-C motif) ligand 1 variant, partial
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: CX3CL1
• NCBI Official Synonym Symbols: NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• NCBI Protein Information: fractalkine
• UniProt Protein Name: Fractalkine
• Protein Family: Fractalkine
• UniProt Gene Name: CX3CL1
• UniProt Synonym Gene Names: FKN; NTT; SCYD1
• UniProt Entry Name: X3CL1_HUMAN

Uniprot Description

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; defense response; chemotaxis; angiogenesis involved in wound healing; leukocyte adhesive activation; macrophage chemotaxis; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response