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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Activating Transcription Factor 3 Polyclonal Antibody | anti-ATF3 antibody

Polyclonal Antibody to Activating Transcription Factor 3 (ATF3)

Gene Names
Atf3; LRG-21
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Activating Transcription Factor 3; Polyclonal Antibody; Polyclonal Antibody to Activating Transcription Factor 3 (ATF3); anti-ATF3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ATF3. It has beenselected for its ability to recognize ATF3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.76mg/ml (varies by lot)
Sequence Length
181
Applicable Applications for anti-ATF3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
ATF3 (Met1~Ser181)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant ATF3 (Met1~Ser181) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064741
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,722 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-3
NCBI Official Synonym Full Names
activating transcription factor 3
NCBI Official Symbol
Atf3
NCBI Official Synonym Symbols
LRG-21
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-3
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-3
UniProt Gene Name
Atf3
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-3

Uniprot Description

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1 H6|1 96.28 cM

Cellular Component: nucleolus; nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; identical protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: cellular response to amino acid starvation; endoplasmic reticulum unfolded protein response; gluconeogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent

Research Articles on ATF3

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Product Notes

The ATF3 atf3 (Catalog #AAA2028352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Activating Transcription Factor 3 (ATF3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Activating Transcription Factor 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the ATF3 atf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Activating Transcription Factor 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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