ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) Recombinant Protein | ATP4a recombinant protein

Recombinant ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)

Cat. Num. AAA2018541

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: ATPase; H+/K+ Exchanging Alpha Polypeptide (ATP4a); Recombinant ATPase; ATP4a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- PELTP YLIYITVSVP LPLGCITILF IELCTDIFPS VSLAYEKAES DIMHLRPRNP KRDRLVNEPL AAYSYFQIGA IQSFAGFADY FTAMAQEGWF PLLCVGLRPQ WEDHHLQDLQ DSYGQEWTFG QRLYQQYTCY TVFFISIEMC QIADVLIRKT RRLSVFQQGF FRNKILVIAI VFQVCIGCFLCYCPGMPNIF NFMPIRFQWW LVPMPFGLLI FVYDEIRKLG VRCC
• Sequence Length: 1025

• Applicable Applications for ATP4a recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Pro786~Cys1014 (Accession # Q91WH7) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 110225337
• NCBI GeneID: 11944
• NCBI Accession #: NP_061201.2
• NCBI GenBank Nucleotide #: NM_018731.2
• UniProt Accession #: Q91WH7; Q64436
• Molecular Weight: 58.7kDa
• NCBI Official Full Name: potassium-transporting ATPase alpha chain 1
• NCBI Official Synonym Full Names: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
• NCBI Official Symbol: Atp4a
• NCBI Protein Information: potassium-transporting ATPase alpha chain 1; proton pump; H+/K+-ATPase alpha; H+K+-transporting alpha 1; gastric H(+)/K(+) ATPase subunit alpha; ATPase, H+/K+ exchanging, alpha polypeptide; ATPase, H+/K+ transporting, alpha polypeptide
• UniProt Protein Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
• Protein Family: Potassium-transporting ATPase
• UniProt Gene Name: Atp4a
• UniProt Entry Name: Q91WH7_MOUSE

Uniprot Description

ATP4A: Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Protein type: Transporter, ion channel; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.10; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Hydrolase

Cellular Component: extracellular space; membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: hydrolase activity; protein heterodimerization activity; metal ion binding; magnesium ion binding; nucleotide binding; sodium:potassium-exchanging ATPase activity; hydrogen:potassium-exchanging ATPase activity; ATP binding

Biological Process: response to drug; proton transport; transport; ATP hydrolysis coupled proton transport; sodium ion transport; pH reduction; regulation of proton transport; ion transport; potassium ion transport