Rabbit anti-Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) Polyclonal Antibody | anti-ALCAM antibody

Polyclonal Antibody to Activated Leukocyte Cell Adhesion Molecule (ALCAM)

Cat. Num. AAA2002659

• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Activated Leukocyte Cell Adhesion Molecule (ALCAM); Polyclonal Antibody; Polyclonal Antibody to Activated Leukocyte Cell Adhesion Molecule (ALCAM); anti-ALCAM antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against ALCAM. It has been selected for its ability to recognize ALCAM in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-WYTVNSAYGD TIVMPCRLDV PQNLMFGKWK YEKPDGSPVF IAFRSSTKKS VQYDDVPEYK DRLSLSENYT LSINNAKISD EKRFVCMLVT EDNVFEAPTL VKVFKQPSKP EIVNRAAFLE TEQLKKLGDC ISRDSYPDGN ITWYRNGKVL QPVDGEVSIL FKKEIDPGTQ LYTMTSSLEY KTTKSDIQMP FTCSVTYYGP SGQKTIYSEQ AIFDIYYPTE QVTIQVLPPK NAIKEGDNIT LQCLGNGNPP PEEFMFYLPG QAEGIRSSNT YTLTDVRRNA TGDYKCSLID QRNMAASTTI TVHYLDLSLN PSGEVTKQIG DTLPVSCTIS ASRNATVVWM KDNIRLRSSP SFSSLHYQDA GNYVCETALQ EVEGLKKRES LTLIVEGKPQ IKMTKKTDPS GLSKTIICHV EGFPKPAIQW TITGSGSVIN QTEESPYING RYYSKIIISP EENVTLTCTA ENQLERTVNS LNVSAISIPE HDEADDISDE NREKVNDQAK
• Sequence Length: 583

• Applicable Applications for anti-ALCAM antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant ALCAM (Trp28~Lys527) expressed in E.coli.
• Cross Reactivity: Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2034740
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:RatTissue))

NCBI and Uniprot Product Information

• NCBI GI #: 13929058
• NCBI GeneID: 79559
• NCBI Accession #: NP_113941.1
• NCBI GenBank Nucleotide #: NM_031753.1
• UniProt Accession #: O35112; O55172
• Molecular Weight: 65,022 Da
• NCBI Official Full Name: CD166 antigen
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: Alcam
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: Alcam

Uniprot Description

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (). Mediates outgrowth and pathfinding for retinal ganglion cell axons ().