Rabbit anti-Human F9 Polyclonal Antibody | anti-F9 antibody

F9 Antibody (Center)

Cat. Num. AAA9205925

• Gene Names: F9; FIX; P19; PTC; HEMB; THPH8
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: F9; Polyclonal Antibody; F9 Antibody (Center); Coagulation factor IX; Christmas factor; Plasma thromboplastin component; PTC; Coagulation factor IXa light chain; Coagulation factor IXa heavy chain; anti-F9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This F9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human F9.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 266-295
• Sequence Length: 461

• Applicable Applications for anti-F9 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(F9 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the F9 antibody detected the F9 protein (arrow).)

Related Product Information for anti-F9 antibody

This gene encodes vitamin K-dependent coagulation factor
IX that circulates in the blood as an inactive zymogen. This factor
is converted to an active form by factor XIa, which excises the
activation peptide and thus generates a heavy chain and a light
chain held together by one or more disulfide bonds. The role of
this activated factor IX in the blood coagulation cascade is to
activate factor X to its active form through interactions with Ca+2
ions, membrane phospholipids, and factor VIII. Alterations of this
gene, including point mutations, insertions and deletions, cause
factor IX deficiency, which is a recessive X-linked disorder, also
called hemophilia B or Christmas disease.

Product Categories/Family for anti-F9 antibody

Cardiovascular; Immunology

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Yang, L., et al. J. Biol. Chem. 285(37):28488-28495(2010)
Kao, C.Y., et al. Thromb. Haemost. 104(2):355-365(2010)
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Arellano, A.R., et al. J. Thromb. Haemost. 8(5):1132-1134(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 4503649
• NCBI GeneID: 2158
• NCBI Accession #: NP_000124.1
• NCBI GenBank Nucleotide #: NM_000133.3
• UniProt Accession #: P00740; Q5FBE1; Q5JYJ8; A8K9N4; F2RM36
• Molecular Weight: 51778
• NCBI Official Full Name: coagulation factor IX preproprotein
• NCBI Official Synonym Full Names: coagulation factor IX
• NCBI Official Symbol: F9
• NCBI Official Synonym Symbols: FIX; P19; PTC; HEMB; THPH8
• NCBI Protein Information: coagulation factor IX
• UniProt Protein Name: Coagulation factor IX
• Protein Family: F9 protein
• UniProt Gene Name: F9
• UniProt Synonym Gene Names: PTC
• UniProt Entry Name: FA9_HUMAN

NCBI Description

This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. [provided by RefSeq, Jul 2008]

Uniprot Description

F9: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB); also known as Christmas disease. Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide. Defects in F9 are the cause of thrombophilia due to factor IX defect (THPH8). A hemostatic disorder characterized by a tendency to thrombosis. Belongs to the peptidase S1 family.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.22; Protease

Chromosomal Location of Human Ortholog: Xq27.1-q27.2

Cellular Component: endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase activity; calcium ion binding

Biological Process: blood coagulation, extrinsic pathway; cellular protein metabolic process; proteolysis; post-translational protein modification; blood coagulation; blood coagulation, intrinsic pathway; peptidyl-glutamic acid carboxylation

Disease: Hemophilia B; Thrombophilia, X-linked, Due To Factor Ix Defect; Coumarin Resistance