Rabbit anti-Rat Acid Sensitive Ion Channel 2b Polyclonal Antibody
Acid Sensitive Ion Channel 2b (ASIC2B, MDEG2, Mammalian Degenerin2)
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Acid Sensitive Ion Channel 2b; Polyclonal Antibody; Acid Sensitive Ion Channel 2b (ASIC2B; MDEG2; Mammalian Degenerin2); Anti -Acid Sensitive Ion Channel 2b (ASIC2B; anti-Acid Sensitive Ion Channel 2b antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Acid Sensitive Ion Channel 2b. No significant sequence homology is detected with other ASICs. Species Sequence Homology
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Applicable Applications for anti-Acid Sensitive Ion Channel 2b antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml with Control Peptide.
Western Blot: 1-10ug/ml (ECL)
Dilution: ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml with Control Peptide.
Western Blot: 1-10ug/ml (ECL)
Immunogen
Synthetic peptide 18aa sequence near the extracellular, N-terminus, of rat MDEG2/ASIC2b.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Acid Sensitive Ion Channel 2b antibody
Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC); (3) mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride.
The mammalian homolog of degenerins (MDEG or MDEG1) is now designated ASIC for Acid Sensing Ion Channels). There are at least three distinct proteins in ASIC family. ASIC1 is identical with human BNAC2 or BNC2. It is expressed in brain and dorsal root ganglions (DRG) cells. It is activated by pH < 7.0. A splice variant of rat ASIC, ASIC-beta, is expressed only in a subset of small and large diameter sensory neurons and absent in sympathetic neurons and CNS. MDEG1/ASIC2, 67% identity with ASIC1, requires more acidic pH than ASIC1 and has slower activation kinetic. MDEG2/ASIC2b, a splice variant of MDEG1, is expressed in both brain and sensory neurons. MDEG2 is activated neither by mutations nor low pH. However, it acts as modulatory subunit when associated with MDEG1 and another H+-activated channel, DRASIC/ASIC3 (Dorsal Root ganglion ASIC). DRASIC is specific for sensory neurons. In response to a drop in pH, DRASIC gives rise to a biphasic currents with poor discrimination between Na+ and K+. This sustained current may be important in pain sensation.
The mammalian homolog of degenerins (MDEG or MDEG1) is now designated ASIC for Acid Sensing Ion Channels). There are at least three distinct proteins in ASIC family. ASIC1 is identical with human BNAC2 or BNC2. It is expressed in brain and dorsal root ganglions (DRG) cells. It is activated by pH < 7.0. A splice variant of rat ASIC, ASIC-beta, is expressed only in a subset of small and large diameter sensory neurons and absent in sympathetic neurons and CNS. MDEG1/ASIC2, 67% identity with ASIC1, requires more acidic pH than ASIC1 and has slower activation kinetic. MDEG2/ASIC2b, a splice variant of MDEG1, is expressed in both brain and sensory neurons. MDEG2 is activated neither by mutations nor low pH. However, it acts as modulatory subunit when associated with MDEG1 and another H+-activated channel, DRASIC/ASIC3 (Dorsal Root ganglion ASIC). DRASIC is specific for sensory neurons. In response to a drop in pH, DRASIC gives rise to a biphasic currents with poor discrimination between Na+ and K+. This sustained current may be important in pain sensation.
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Product Notes
The Acid Sensitive Ion Channel 2b (Catalog #AAA610776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acid Sensitive Ion Channel 2b (ASIC2B, MDEG2, Mammalian Degenerin2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Acid Sensitive Ion Channel 2b can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml with Control Peptide. Western Blot: 1-10ug/ml (ECL). Researchers should empirically determine the suitability of the Acid Sensitive Ion Channel 2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acid Sensitive Ion Channel 2b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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