Rabbit anti-Human ALPP Polyclonal Antibody | anti-ALPP antibody

ALPP, NT (ALPP, PLAP, Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1) (MaxLight 650)

Cat. Num. AAA6273067

• Gene Names: ALPP; ALP; PALP; PLAP; PLAP-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ALPP; Polyclonal Antibody; NT (ALPP; PLAP; Alkaline phosphatase; placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1) (MaxLight 650); Placental alkaline phosphatase 1; anti-ALPP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALPP.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ALPP antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 56-83 in the N-terminal region of human ALPP, UniProt Accession #P05187.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HepG2 cell line lysates (35ug/lane) usingMBS6002536. This demonstrates that MBS6002536 detected the ALPP protein (arrow).)

Western Blot (WB)

(Western Blot analysis of ALPP (arrow) 293 cell lysates (2 ug/lane) using MBS6002536 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPP gene.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human placenta tissue using MBS6002536 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-ALPP antibody

Antibodies; Enzymes; Phosphatase

References

Stec, B., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010): Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009). Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009). Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009). Roberson, J.R., et al. Pediatr Blood Cancer 51(6):840-842(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 94721246
• NCBI GeneID: 250
• NCBI Accession #: NP_001623.3
• NCBI GenBank Nucleotide #: NM_001632.3
• UniProt Accession #: P05187; P05188; P06861; Q53S78; Q96DB7
• Molecular Weight: 57,954 Da
• NCBI Official Full Name: alkaline phosphatase, placental type preproprotein
• NCBI Official Synonym Full Names: alkaline phosphatase, placental
• NCBI Official Symbol: ALPP
• NCBI Official Synonym Symbols: ALP; PALP; PLAP; PLAP-1
• NCBI Protein Information: alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme
• UniProt Protein Name: Alkaline phosphatase, placental type
• Protein Family: Alkaline phosphatase
• UniProt Gene Name: ALPP
• UniProt Synonym Gene Names: PLAP; PLAP-1
• UniProt Entry Name: PPB1_HUMAN

NCBI Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Protein type: Cell surface; Membrane protein, integral; Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein); Motility/polarity/chemotaxis; Membrane protein, GPI anchor; EC 3.1.3.1

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cell surface; plasma membrane; integral to membrane

Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding

Biological Process: dephosphorylation