Western Blot (WB)
(Sample Data: Representative western blot of the acetylated protein profile with Anti-Acetylated Lysine (1:1000) using Hela cell lysate (A) and Hela cell lysate plus additional acetylated BSA (10 ng/ul) in the primary antibody reaction (B).)
Rabbit Acetylated Lysine Polyclonal Antibody
Anti-Acetylated Lysine
Reactivity
Pan-specific antibodyNo reaction to non-acetylated proteins, peptides.
Applications
Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
By affinity chromatography on an acetyl lysine agarose column
Synonyms
Acetylated Lysine; Polyclonal Antibody; Anti-Acetylated Lysine; anti-Acetylated Lysine antibody
Host
Rabbit
Reactivity
Pan-specific antibody
No reaction to non-acetylated proteins, peptides.
No reaction to non-acetylated proteins, peptides.
Clonality
Polyclonal
Specificity
Recognizes acetylated lysine residues in proteins and peptide, including acetylated histone, acetylated BSA and acetylated MBP.
Purity/Purification
By affinity chromatography on an acetyl lysine agarose column
Form/Format
PBS, 50% glycerol, pH7.
Applicable Applications for anti-Acetylated Lysine antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Immunogen
Acetylated lysine-KLH conjugates
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot of the acetylated protein profile with Anti-Acetylated Lysine (1:1000) using Hela cell lysate (A) and Hela cell lysate plus additional acetylated BSA (10 ng/ul) in the primary antibody reaction (B).)
Western Blot (WB)
(Sample Data: Representative western blot of the acetylated protein profile with Anti-Acetylated Lysine (1:1000) using Hela cell lysate (A) and Hela cell lysate plus additional acetylated BSA (10 ng/ul) in the primary antibody reaction (B).)
Related Product Information for anti-Acetylated Lysine antibody
Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Acetylation on lysine residues is one example of post-translational modification and is performed by a variety of acetyl transferases in the cell. Histone acetyl-transferases (HATs) are universal regulators of eukaryotic transcriptional activity and emerging therapeutic targets for human diseases (1). The Acetylated Lysine antibody detects Acetylation on lysine residues of proteins and peptide.
Product Categories/Family for anti-Acetylated Lysine antibody
References
1. Mischerikow, N. et al: Targeted large-scale analysis of protein acetylation. Proteomics. 2011 Feb;11(4):571-89.
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Product Notes
The Acetylated Lysine (Catalog #AAA516055) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Acetylated Lysine reacts with Pan-specific antibody No reaction to non-acetylated proteins, peptides. and may cross-react with other species as described in the data sheet. AAA Biotech's Acetylated Lysine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the Acetylated Lysine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acetylated Lysine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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