Rabbit anti-Mouse Integrin beta 1 Polyclonal Antibody | anti-ITGB1 antibody

Anti-Integrin beta 1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103501

• Gene Names: Itgb1; CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik; ENSMUSG00000051907
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Integrin beta 1; Polyclonal Antibody; Anti-Integrin beta 1 Antibody; Rabbit Polyclonal; Integrin beta 1/ITGB1 Antibody; Rabbit PAb; Antigen Affinity Purified; integrin; beta 1 (fibronectin receptor; beta polypeptide; antigen CD29 includes MDF2; MSK12); Anti-4633401G24Rik Antibody; Anti-AA409975 Antibody; Anti-AA960159 Antibody; Anti-CD29 Antibody; Anti-ENSMUSG00000051907 Antibody; Anti-Fnrb Antibody; Anti-Gm9863 Antibody; Anti-gpIIa Antibody; anti-ITGB1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Integrin beta 1/ITGB1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ITGB1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse Integrin beta 1/ITGB1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Integrin beta 1/ITGB1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ITGB1 rabbit polyclonal antibody at 1:500 dilution Lane A: Raw264.7 Whole Cell Lysate Lane B: Mouse heart tissue lysate Lane C: Mouse kidney tissue lysate Lane D: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:88 kDa)

Immunoprecipitation (IP)

(LCP1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-LCP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-LCP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa Observed band size :70 kDa)

Related Product Information for anti-ITGB1 antibody

ITGB1 (Integrin Subunit Beta 1) is a Protein Coding gene. This gene encodes a beta subunit, which is a type 1 transmembrane protein of the integrin beta chain family. ITGB1 is a heterodimeric cell-surface receptor involved in cell functions such as proliferation, migration, invasion, and survival. ITGB1 has been recognized to play a major role in tumor growth, invasion, and metastasis. Using luciferase assays, the researcher identified ITGB1 as a direct target of miR-134. ITGB1 is a direct target of miR-493-5p suggesting that ITGB1 and miR-493-5p may have potential prognostic value and may be useful as tumor biomarkers for the identification of NSCLC patients. Diseases associated with ITGB1 include Gallbladder Cancer and Breast Cancer.

Product Categories/Family for anti-ITGB1 antibody

Cardiac Stem Cell Markers; Actin Dynamics Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 124964
• NCBI GeneID: 16412
• UniProt Accession #: P09055; Q3TIW5; Q3TWH6; Q60993; Q61126; Q8BTU0; Q8BVU1; F6R105
• Molecular Weight: 88,700 Da
• NCBI Official Full Name: Integrin beta-1
• NCBI Official Synonym Full Names: integrin beta 1 (fibronectin receptor beta)
• NCBI Official Symbol: Itgb1
• NCBI Official Synonym Symbols: CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik; ENSMUSG00000051907
• NCBI Protein Information: integrin beta-1; VLA-4 subunit beta; fibronectin receptor subunit beta
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: Itgb1
• UniProt Entry Name: ITB1_MOUSE

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Membrane protein, integral; Cell adhesion; Receptor, misc.; Motility/polarity/chemotaxis; Cell surface

Cellular Component: focal adhesion; cell surface; dendritic spine; integral to membrane; acrosome; intercellular junction; lipid raft; cell projection; adherens junction; hemidesmosome; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; basement membrane; synapse; cytoplasmic vesicle; neuromuscular junction; cell junction; integrin complex; receptor complex; sarcolemma; endosome; filopodium; external side of plasma membrane

Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; receptor activity; actin binding; alpha-actinin binding; peptide binding; protein kinase binding; collagen binding; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; protein complex binding; cell adhesion molecule binding; glycoprotein binding; kinase binding; receptor binding

Biological Process: positive regulation of apoptosis; axon extension; regulation of cell cycle; multicellular organismal development; cell-matrix adhesion; cell fate specification; positive regulation of endocytosis; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; germ cell migration; positive regulation of cell proliferation; tissue homeostasis; visual learning; cell adhesion; negative regulation of cell projection organization and biogenesis; neurite development; integrin-mediated signaling pathway; cardiac muscle development; cell migration; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; formation of radial glial scaffolds; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; negative regulation of cell differentiation; negative regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; negative regulation of Rho protein signal transduction; calcium-independent cell-matrix adhesion; stress fiber formation; positive regulation of neuron differentiation; leukocyte tethering or rolling; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; positive regulation of cell migration