Rabbit Acetyl-Histone H2B Polyclonal Antibody | anti-H2B antibody

Acetyl-Histone H2B (Lys5) Rabbit pAb

Cat. Num. AAA4757691

• Reactivity: Human, Mouse, Rat, A wide range of other species
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: Acetyl-Histone H2B; Polyclonal Antibody; Acetyl-Histone H2B (Lys5) Rabbit pAb; anti-H2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, A wide range of other species
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-H2B antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
• Application Notes: WB: 1:500-1:2000; IHC1: 1:50-1:200; IP: 1:50-1:100
• Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B
• Conjugation: Unconjugated
• Modification: Acetylated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot - Acetyl-Histone H2B-K5 pAb Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K5 antibody at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hoursSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Exposure time: 60s.)

Immunofluorescence (IF)

(Immunofluorescence - Acetyl-Histone H2B-K5 pAb Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence - Acetyl-Histone H2B-K5 pAb Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence - Acetyl-Histone H2B-K5 pAb Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-H2B antibody

Primary antibody