Western Blot (WB)
(Western blot analysis of extracts of various cell lines using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:1000. HeLa cells were treated by TSA (1 uM) at 37 degree C for 18 hours. C2C12 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.)
Rabbit Acetyl-Histone H2B-K12 Polyclonal Antibody | anti-H2B-K12 antibody
Acetyl-Histone H2B-K12 Polyclonal Antibody
IHC: 1:50-1:200
IF: 1:50-1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:1000. HeLa cells were treated by TSA (1 uM) at 37 degree C for 18 hours. C2C12 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:1000. HeLa cells were treated by TSA (1 uM) at 37 degree C for 18 hours. C2C12 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human colon using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human colon using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat ovary using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat ovary using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse testis using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse testis using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:200 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 Polyclonal Antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. [provided by RefSeq, Dec 2014]
Uniprot Description
H2B2E: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2B family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: nucleoplasm; extracellular space; nucleosome; nucleus
Molecular Function: DNA binding; protein heterodimerization activity
Biological Process: nucleosome assembly; defense response to Gram-positive bacterium; establishment and/or maintenance of chromatin architecture; innate immune response in mucosa; antibacterial humoral response
Research Articles on H2B-K12
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Product Notes
The H2B-K12 hist2h2be (Catalog #AAA2565335) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acetyl-Histone H2B-K12 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Acetyl-Histone H2B-K12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the H2B-K12 hist2h2be for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acetyl-Histone H2B-K12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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