Rabbit Acetyl-Histone H2B-K15 Polyclonal Antibody | anti-H2B-K15 antibody
Acetyl-Histone H2B-K15 Polyclonal Antibody
IHC: 1:50-1:200
IF: 1:50-1:100
Western Blot (WB)
(Western blot analysis of extracts of various cell lines using Acetyl-Histone H2B-K15 Polyclonal Antibody at dilution of 1:1000. HeLa cells were treated by TSA (1 uM) at 37 degree C for 18 hours. NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat lung using Acetyl-Histone H2B-K15 Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human breast cancer using Acetyl-Histone H2B-K15 Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human gastric cancer using Acetyl-Histone H2B-K15 Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse brain using Acetyl-Histone H2B-K15 Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 Polyclonal Antibody at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 Polyclonal Antibody at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 Polyclonal Antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37 degree C for 18 hours. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. [provided by RefSeq, Dec 2014]
Uniprot Description
H2B2E: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2B family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: nucleoplasm; extracellular space; nucleosome; nucleus
Molecular Function: DNA binding; protein heterodimerization activity
Biological Process: nucleosome assembly; defense response to Gram-positive bacterium; establishment and/or maintenance of chromatin architecture; innate immune response in mucosa; antibacterial humoral response
