Rabbit Acetyl CoA Carboxylase Polyclonal Antibody

Acetyl CoA Carboxylase (ACC1)

Cat. Num. AAA616976

• Reactivity: Bovine, Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: Acetyl CoA Carboxylase; Polyclonal Antibody; Acetyl CoA Carboxylase (ACC1); Anti -Acetyl CoA Carboxylase (ACC1); anti-Acetyl CoA Carboxylase antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of all isoforms of human acetyl CoA carboxylase protein at ~280kD. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-Acetyl CoA Carboxylase antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence.; Dilution: Flow Cytometry: 1:25; Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:50. Antigen retrieval with citrate buffer is recommended.; Immunoprecipitation: 1:50; Immunofluorescence (IF-IC): 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Lys557 of human acetyl CoA carboxylase a1 conjugated to KLH. MW of Antigen: 280kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from HEK293, HeLa, A431, NIH/3T3, L929, C6, H-4-II-E and BAEC cells, using MBS616976 Acetyl CoA Carboxylase Antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of untreated HeLa cells, using MBS616976 cetyl CoA Carboxylase Antibody (right) compared to a nonspecific negative control antibody (left).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MBS616976 in the presence of control peptide (left) or AcetylCoA Carboxylase Blocking Peptide (right).)

Testing Data

(Confocal microscopic images of A431 cells showing cytoplasmic stain with MBS616976 (A) compared to an isotype control (B).)

Related Product Information for anti-Acetyl CoA Carboxylase antibody

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACCa is the predominant isoform in liver, adipocytes and mammary gland, while the 280kD ACCb is the major isoform in skeletal muscle and heart. Phosphorylation by AMPK at Ser79, or by PKA at Ser1200, inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs.

Product Categories/Family for anti-Acetyl CoA Carboxylase antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 7619891
• NCBI Official Full Name: acetyl-CoA carboxylase