Rabbit anti-Mouse Stearoyl CoA Desaturase 1 Monoclonal Antibody | anti-scd antibody

Stearoyl CoA Desaturase 1 (SCD1)

Cat. Num. AAA602285

• Gene Names: scd; scd
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: Stearoyl CoA Desaturase 1; Monoclonal Antibody; Stearoyl CoA Desaturase 1 (SCD1); Anti -Stearoyl CoA Desaturase 1 (SCD1); anti-scd antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9j153
• Specificity: Recognizes endogenous levels of total mouse Stearoyl CoA Desaturase 1 at 37kD.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-scd antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunoflourescence.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50; Immunohistochemistry (Paraffin): 1:200; Immunoflourescence (IF-IC): 1:100
• Immunogen: Synthetic peptide corresponding to residues surronding Leu35 of mouse Stearoyl CoA Desaturase 1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of 3T3-L1 cell lysates using MBS602285)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded 3T3-L1 cells undifferentiated (left) or differentiated (right) using MBS602285)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse lung using MBS602285 (left) or without primary antibody (right).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin- embedded mouse brown fat using MBS602285 in the presence of control peptide (left) or antigen-specific peptide (right).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NIH/3T3-L1 cells, using MBS602285 (red) showing cytoplasmic localization in differentiated cells.)

Related Product Information for anti-scd antibody

Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl-CoA and stearoyl- CoA to palmitoleoyl-CoA (16:1) and oleoyl-CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5).

Product Categories/Family for anti-scd antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 38488714
• NCBI GeneID: 386661
• NCBI Accession #: NP_942110.1
• NCBI GenBank Nucleotide #: NM_198815.1
• UniProt Accession #: Q804A7
• Molecular Weight: 37,542 Da
• NCBI Official Full Name: stearoyl-CoA desaturase 5
• NCBI Official Synonym Full Names: stearoyl-CoA desaturase (delta-9-desaturase)
• NCBI Official Symbol: scd
• NCBI Official Synonym Symbols: scd
• NCBI Protein Information: stearoyl-CoA desaturase 5; SCD1
• UniProt Protein Name: Stearoyl-CoA desaturase
• Protein Family: Acyl-CoA desaturase
• UniProt Gene Name: scd
• UniProt Entry Name: Q804A7_DANRE

Uniprot Description

Cofactor: Iron

By similarity. RuleBase RU000581

Domain: The histidine box domains may contain the active site and/or be involved in metal ion binding

By similarity. RuleBase RU000581

Sequence similarities: Belongs to the fatty acid desaturase family. RuleBase RU000581