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Western Blot (WB) (Western blot analysis of HOXC13 using HepG2 whole cell lysates)

Rabbit HOXC13 Polyclonal Antibody | anti-HOXC13 antibody

HOXC13 Antibody

Gene Names
HOXC13; HOX3; ECTD9; HOX3G
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
HOXC13; Polyclonal Antibody; HOXC13 Antibody; ECTD9; Homeo box 3G; Homeo box C13; homeobox C13; Homeobox protein Hox 3G; Homeobox protein Hox-3G; Homeobox protein Hox-C13; Homeobox protein Hox-C13a; Hox-3G; HOX3; HOX3G; Hoxc13; HXC13; HXC13_HUMAN; NUP98; NUP98/HOXC13; anti-HOXC13 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HOXC13 antibody detects endogenous levels of total HOXC13
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
330
Applicable Applications for anti-HOXC13 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
ELISA(peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human HOXC13, corresponding to a region within C-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of HOXC13 using HepG2 whole cell lysates)

Western Blot (WB) (Western blot analysis of HOXC13 using HepG2 whole cell lysates)

Western Blot (WB)

(Western blot analysis of extracts from c476, using HOXC13 Antibody. Lane 1 was treated with the blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from c476, using HOXC13 Antibody. Lane 1 was treated with the blocking peptide.)

Immunohistochemistry (IHC)

(MBS9609899 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9609899 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35 kDa
NCBI Official Full Name
homeobox protein Hox-C13
NCBI Official Synonym Full Names
homeobox C13
NCBI Official Symbol
HOXC13
NCBI Official Synonym Symbols
HOX3; ECTD9; HOX3G
NCBI Protein Information
homeobox protein Hox-C13
UniProt Protein Name
Homeobox protein Hox-C13
Protein Family
UniProt Gene Name
HOXC13
UniProt Synonym Gene Names
HOX3G

NCBI Description

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene may play a role in the development of hair, nail, and filiform papilla. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor which plays a role in hair follicle differentiation. Regulates FOXQ1 expression and that of other hair-specific genes ().

Research Articles on HOXC13

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Product Notes

The HOXC13 hoxc13 (Catalog #AAA9609899) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HOXC13 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HOXC13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200 ELISA(peptide): 1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the HOXC13 hoxc13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOXC13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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