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Western Blot (WB) (Western blot analysis of extracts of HEK-293 cell lines, using HCFC1 antibody.)

Rabbit HCFC1 Polyclonal Antibody | anti-HCFC1 antibody

HCFC1 (Host Cell Factor C1, CFF, HCF1, HFC1, MRX3, VCAF, HCF-1) (FITC)

Gene Names
HCFC1; CFF; HCF1; HFC1; MRX3; VCAF; HCF-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
HCFC1; Polyclonal Antibody; HCFC1 (Host Cell Factor C1; CFF; HCF1; HFC1; MRX3; VCAF; HCF-1) (FITC); anti-HCFC1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes HCFC1. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-HCFC1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:200
WB: 1:500-1:2000
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human HCFC1.
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HEK-293 cell lines, using HCFC1 antibody.)

Western Blot (WB) (Western blot analysis of extracts of HEK-293 cell lines, using HCFC1 antibody.)
Product Categories/Family for anti-HCFC1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
208,732 Da
NCBI Official Full Name
host cell factor 1
NCBI Official Synonym Full Names
host cell factor C1 (VP16-accessory protein)
NCBI Official Symbol
HCFC1
NCBI Official Synonym Symbols
CFF; HCF1; HFC1; MRX3; VCAF; HCF-1
NCBI Protein Information
host cell factor 1; HCF; C1 factor; VP16 accessory protein
UniProt Protein Name
Host cell factor 1
Protein Family
UniProt Gene Name
HCFC1
UniProt Synonym Gene Names
HCF1; HFC1; HCF; HCF-1
UniProt Entry Name
HCFC1_HUMAN

NCBI Description

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HCFC1: Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; cell soma; mitochondrion; membrane; cytoplasm; nucleus; histone acetyltransferase complex

Molecular Function: identical protein binding; protein binding; transcription coactivator activity; chromatin binding; histone acetyltransferase activity (H4-K16 specific); transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; establishment and/or maintenance of chromatin architecture; protein stabilization; regulation of transcription, DNA-dependent; organelle organization and biogenesis; reactivation of latent virus; regulation of protein complex assembly; positive regulation of cell cycle; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell cycle

Disease: Methylmalonic Acidemia And Homocysteinemia, Cblx Type

Research Articles on HCFC1

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Product Notes

The HCFC1 hcfc1 (Catalog #AAA6419932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HCFC1 (Host Cell Factor C1, CFF, HCF1, HFC1, MRX3, VCAF, HCF-1) (FITC) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HCFC1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:200 WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HCFC1 hcfc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCFC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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