Rabbit ACCN2 Polyclonal Antibody | anti-ACCN2 antibody

ACCN2 Polyclonal Antibody

Cat. Num. AAA3012188

• Gene Names: ASIC1; ASIC; ACCN2; BNaC2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified.
• Synonyms: ACCN2; Polyclonal Antibody; ACCN2 Polyclonal Antibody; ASIC1; BNaC2; BNAC2; Acid sensing ion channel 1; Acid sensing ion channel 1a protein; Acid sensing ion channel; Acid-sensing ion channel 1; AI843610; Amiloride sensitive cation channel 2 neuronal; Amiloride-sensitive cation channel 2; ASIC; ASIC1A; B530003N02Rik; Brain sodium channel 2; Cation channel amiloride sensitive neuronal 2; hBNaC2; neuronal; anti-ACCN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ACCN2 polyclonal antibody detects endogenous levels of ACCN2 protein.
• Purity/Purification: Peptide affinity purified.
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 562

• Applicable Applications for anti-ACCN2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Synthetic peptide within human ACCN2 aa 460-500.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of ACCN2 on SH-SY-5Y cell lysate using anti-ACCN2 antibody at 1/2,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-ACCN2 antibody

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.Isoform 1 does not display proton-gated cation channel activity.

NCBI and Uniprot Product Information

• NCBI GI #: 378744190
• NCBI GeneID: 41
• NCBI Accession #: NP_001243759.1
• NCBI GenBank Nucleotide #: NP_001243759.1
• UniProt Accession #: P78348; Q6NXK8; P55926
• Molecular Weight: 60kDa
• NCBI Official Full Name: acid-sensing ion channel 1 isoform c
• NCBI Official Synonym Full Names: acid sensing ion channel subunit 1
• NCBI Official Symbol: ASIC1
• NCBI Official Synonym Symbols: ASIC; ACCN2; BNaC2
• NCBI Protein Information: acid-sensing ion channel 1
• UniProt Protein Name: Acid-sensing ion channel 1
• UniProt Gene Name: ASIC1
• UniProt Synonym Gene Names: ACCN2; BNAC2; ASIC1; BNaC2
• UniProt Entry Name: ASIC1_HUMAN

NCBI Description

This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

Function: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca2+, Li+ and K+. Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear

By similarity.

Subunit structure: Homotrimer or heterotrimer with other ASIC proteins

By similarity. Interacts with STOM and ASIC2

By similarity. Interacts with PRKCABP. Ref.8 Ref.10

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Note: Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4

By similarity. Ref.10

Tissue specificity: Expressed in most or all neurons.

Post-translational modification: Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.

Miscellaneous: Potentiated by Ca2+, Mg2+, Ba2+ and multivalent cations. Inhibited by anti-inflammatory drugs like salicylic acid

By similarity. Potentiated by FMRFamide-related neuropeptides. PH dependence may be regulated by serine proteases.

Sequence similarities: Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC1 subfamily. [View classification]