Rat ABCA1 Monoclonal Antibody | anti-ABCA1 antibody

RAT ANTI MOUSE ABCA1:RPE

Cat. Num. AAA219656

• Gene Names: Abca1; Abc1; ABC-1
• Applications: Flow Cytometry, Functional Assay
• Synonyms: ABCA1; Monoclonal Antibody; RAT ANTI MOUSE ABCA1:RPE; anti-ABCA1 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 5A1-1422
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 2261

• Applicable Applications for anti-ABCA1 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1ml distilled water ; Perservative Stabilisers
• Immunogen: ABCA1 transfected HeLa cells.; Buffer Solution
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:RPE)

Testing Data

(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:Alexa Fluor 488 (MBS219757A488))

Testing Data

(Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:FITC (MBS219647))

Related Product Information for anti-ABCA1 antibody

Rat anti Mouse ABCA1 antibody, clone 5A1-1422 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease. Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency.

NCBI and Uniprot Product Information

• NCBI GI #: 90568038
• NCBI GeneID: 11303
• NCBI Accession #: NP_038482.3
• NCBI GenBank Nucleotide #: NM_013454.3
• UniProt Accession #: P41233; B1AWZ8
• Molecular Weight: 253,912 Da
• NCBI Official Full Name: ATP-binding cassette sub-family A member 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family A (ABC1), member 1
• NCBI Official Symbol: Abca1
• NCBI Official Synonym Symbols: Abc1; ABC-1
• NCBI Protein Information: ATP-binding cassette sub-family A member 1; ATP-binding cassette 1; ATP-binding cassette transporter 1
• UniProt Protein Name: ATP-binding cassette sub-family A member 1
• Protein Family: ABC transporter A family
• UniProt Gene Name: Abca1
• UniProt Synonym Gene Names: Abc1; ABC-1; ATP-binding cassette 1
• UniProt Entry Name: ABCA1_MOUSE

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. In humans, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in the human gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.

Protein type: Membrane protein, integral; Transporter, ABC family; Membrane protein, multi-pass; Vesicle; Transporter

Cellular Component: Golgi apparatus; cell surface; intracellular membrane-bound organelle; membrane; integral to plasma membrane; endocytic vesicle; perinuclear region of cytoplasm; integral to membrane; plasma membrane; phagocytic vesicle; lipid raft; external side of plasma membrane

Molecular Function: apolipoprotein A-I receptor activity; transporter activity; ATPase activity; nucleotide binding; apolipoprotein binding; ATPase binding; small GTPase binding; apolipoprotein A-I binding; protein binding; syntaxin binding; phospholipid transporter activity; ATPase activity, coupled to transmembrane movement of substances; cholesterol transporter activity; anion transmembrane transporter activity; ATP binding; receptor binding

Biological Process: endosome transport; platelet dense granule organization and biogenesis; phospholipid efflux; transport; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; interleukin-1 beta secretion; transmembrane transport; phospholipid homeostasis; peptide secretion; cholesterol metabolic process; response to high density lipoprotein stimulus; regulation of Cdc42 protein signal transduction; cholesterol transport; intracellular cholesterol transport; cholesterol efflux; lipoprotein metabolic process; phospholipid translocation; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; lipoprotein biosynthetic process; lysosome organization and biogenesis; phospholipid transport; phagocytosis, engulfment