Rabbit anti-Human, Mouse ASIC1 Polyclonal Antibody | anti-ASIC1 antibody

ASIC1 Antibody

Cat. Num. AAA7108780

• Gene Names: ASIC1; ASIC; ACCN2; BNaC2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: ASIC1; Polyclonal Antibody; ASIC1 Antibody; Acid-sensing ion channel 1; Amiloride-sensitive cation channel 2; neuronal; Brain sodium channel 21; ACCN2; BNAC2; anti-ASIC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 528

• Applicable Applications for anti-ASIC1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IF: 1:50-1:200
• Immunogen: Recombinant Human Acid-sensing ion channel 1 protein (195-297AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse brain tissueAll lanes: ASIC1 antibody at 4.24ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60, 65, 63 KDaObserved band size: 60 KDa)

Immunofluorescence (IF)

(Immunofluorescence staining of HepG2 cells with MBS7108780 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Related Product Information for anti-ASIC1 antibody

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.

Product Categories/Family for anti-ASIC1 antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 21536349
• NCBI GeneID: 41
• NCBI Accession #: NP_001086.2
• NCBI GenBank Nucleotide #: NM_001095.3
• UniProt Accession #: P78348; P78349; Q96CV2; A3KN86; E5KBL7
• Molecular Weight: 62,700 Da
• NCBI Official Full Name: acid-sensing ion channel 1 isoform b
• NCBI Official Synonym Full Names: acid sensing ion channel subunit 1
• NCBI Official Symbol: ASIC1
• NCBI Official Synonym Symbols: ASIC; ACCN2; BNaC2
• NCBI Protein Information: acid-sensing ion channel 1
• UniProt Protein Name: Acid-sensing ion channel 1
• Protein Family: Acid-sensing ion channel
• UniProt Gene Name: ASIC1
• UniProt Synonym Gene Names: ACCN2; BNAC2; BNaC2

NCBI Description

This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.