Rabbit ACAT1 Polyclonal Antibody | anti-ACAT1 antibody

ACAT1 Rabbit Polyclonal

Cat. Num. AAA766304

• Gene Names: ACAT1; T2; MAT; ACAT; THIL
• Reactivity: Human, Mouse; other species are not tested
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: ACAT1; Polyclonal Antibody; ACAT1 Rabbit Polyclonal; ACAT; Acetoacetyl CoA thiolase; MAT; T2; THIL; anti-ACAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; other species are not tested
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Please decide the specificity by homology
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 427

• Applicable Applications for anti-ACAT1 antibody: ELISA, WB, IHC
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Immunogen: Acetyl-Coenzyme A acetyltransferase 1
• Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Calculated MW: 40 kDa
• Preparation and Storage: Store at -20° for 24 months (Avoid repeated freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using MBS766304 (ACAT1 antibody) at dilution of 1:100)

Western Blot (WB)

(human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with MBS766304 (ACAT1 antibody) at dilution of 1:1000)

Related Product Information for anti-ACAT1 antibody

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of cetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

NCBI and Uniprot Product Information

• NCBI GI #: 4557237
• NCBI GeneID: 38
• NCBI Accession #: NP_000010.1
• NCBI GenBank Nucleotide #: NM_000019.3
• NCBI Official Full Name: acetyl-CoA acetyltransferase, mitochondrial
• NCBI Official Synonym Full Names: acetyl-CoA acetyltransferase 1
• NCBI Official Symbol: ACAT1
• NCBI Official Synonym Symbols: T2; MAT; ACAT; THIL
• NCBI Protein Information: acetyl-CoA acetyltransferase, mitochondrial
• UniProt Protein Name: Acetyl-CoA acetyltransferase, mitochondrial
• UniProt Gene Name: ACAT1
• UniProt Synonym Gene Names: ACAT; MAT
• UniProt Entry Name: THIL_HUMAN

NCBI Description

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Uniprot Description

ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - tryptophan; EC 2.3.1.9; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Lipid Metabolism - fatty acid; Acetyltransferase; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - propanoate; Mitochondrial; Amino Acid Metabolism - lysine degradation; Lipid Metabolism - synthesis and degradation of ketone bodies

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: acetyl-CoA C-acetyltransferase activity; coenzyme binding; enzyme binding; metal ion binding; protein homodimerization activity

Biological Process: brain development; branched chain family amino acid catabolic process; ketone body biosynthetic process; ketone body catabolic process; liver development; protein homooligomerization; response to hormone stimulus; response to organic cyclic substance; response to starvation

Disease: Alpha-methylacetoacetic Aciduria