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Immunohistochemistry (IHC) (Anti-MED17/TRAP80 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.2 ug/ml.)

Mouse anti-Human MED17/TRAP80 Monoclonal Antibody | anti-MED17/TRAP80 antibody

Anti-MED17/TRAP80 Antibody (clone 4D4)

Gene Names
MED17; SRB4; CRSP6; CRSP77; DRIP80; TRAP80
Reactivity
Human
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified from ascites by Protein A
Synonyms
MED17/TRAP80; Monoclonal Antibody; Anti-MED17/TRAP80 Antibody (clone 4D4); Rabbit Polyclonal (IgG) to Human MED17/TRAP80; MED17; CRSP77; CRSP complex subunit 6; CRSP6; DRIP77; Mediator complex subunit 17; TRAP80; ARC77; DRIP80; anti-MED17/TRAP80 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
4D4
Specificity
Human MED17
Purity/Purification
Purified from ascites by Protein A
Form/Format
PBS, pH 7.4
Sequence Length
651
Applicable Applications for anti-MED17/TRAP80 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 10 ug/ml
IHC-P: 1.2-5 ug/ml
WB: 1:500-1:1000
Immunogen
CRSP6 (AAH21101, 551 a.a. ~ 652 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C or lower.
Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MED17/TRAP80 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.2 ug/ml.)

Immunohistochemistry (IHC) (Anti-MED17/TRAP80 antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.2 ug/ml.)

Immunohistochemistry (WB)

(Anti-MED17/TRAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.2 ug/ml.)

Immunohistochemistry (WB) (Anti-MED17/TRAP80 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.2 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CRSP6 on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to CRSP6 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

( Western blot of CRSP6 expression in HeLa NE.)

Western Blot (WB) ( Western blot of CRSP6 expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CRSP6 is approximately 0.3 ng/ml as a capture antibody.)

ELISA (EIA) (Detection limit for recombinant GST tagged CRSP6 is approximately 0.3 ng/ml as a capture antibody.)
Related Product Information for anti-MED17/TRAP80 antibody
Mediator complex, a coactivator involved in the regulated transcriptionof nearly all RNA polymerase II-dependent genes. Mediator functions as abridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,951 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 17
NCBI Official Synonym Full Names
mediator complex subunit 17
NCBI Official Symbol
MED17
NCBI Official Synonym Symbols
SRB4; CRSP6; CRSP77; DRIP80; TRAP80
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 17
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 17
UniProt Gene Name
MED17
UniProt Synonym Gene Names
ARC77; CRSP6; DRIP77; DRIP80; TRAP80; ARC77; CRSP complex subunit 6; Trap80; DRIP80

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Research Articles on MED17/TRAP80

Similar Products

Product Notes

The MED17/TRAP80 med17 (Catalog #AAA2400630) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MED17/TRAP80 Antibody (clone 4D4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MED17/TRAP80 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 10 ug/ml IHC-P: 1.2-5 ug/ml WB: 1:500-1:1000. Researchers should empirically determine the suitability of the MED17/TRAP80 med17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED17/TRAP80, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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