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Western Blot (WB) (Western Blot: Sample: Recombinant CGa, Human.)

Rabbit anti-Human Chorionic Gonadotropin Alpha Polypeptide (CGa) Polyclonal Antibody | anti-CGa antibody

Polyclonal Antibody to Chorionic Gonadotropin Alpha Polypeptide (CGa)

Gene Names
CGA; HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Chorionic Gonadotropin Alpha Polypeptide (CGa); Polyclonal Antibody; Polyclonal Antibody to Chorionic Gonadotropin Alpha Polypeptide (CGa); anti-CGa antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CGa. It has been selected for its ability to recognize CGa in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.7mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-APDVQD CPECTLQENP FFSQPGAPIL QCMGCCFSRA YPTPLRSKKT
Applicable Applications for anti-CGa antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CGa (Ala25~Ser116) expressed in E.coli.
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant CGa, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant CGa, Human.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,075 Da
NCBI Official Full Name
glycoprotein hormones alpha chain isoform 2
NCBI Official Synonym Full Names
glycoprotein hormones, alpha polypeptide
NCBI Official Symbol
CGA
NCBI Official Synonym Symbols
HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
NCBI Protein Information
glycoprotein hormones alpha chain
UniProt Protein Name
Glycoprotein hormones alpha chain
Protein Family
UniProt Gene Name
CGA
UniProt Synonym Gene Names
CG-alpha; FSH-alpha; LSH-alpha; TSH-alpha

NCBI Description

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CGA: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q14.3

Cellular Component: extracellular region; Golgi lumen

Molecular Function: hormone activity; protein binding

Biological Process: cell-cell signaling; G-protein coupled receptor signaling pathway; peptide hormone processing; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity; regulation of transcription from RNA polymerase II promoter; signal transduction

Research Articles on CGa

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Product Notes

The CGa cga (Catalog #AAA2006528) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Chorionic Gonadotropin Alpha Polypeptide (CGa) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Chorionic Gonadotropin Alpha Polypeptide (CGa) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CGa cga for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-APDVQD CPECTLQENP FFSQPGAPIL QCMGCCFSRA YPTPLRSKKT. It is sometimes possible for the material contained within the vial of "Chorionic Gonadotropin Alpha Polypeptide (CGa), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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