Rabbit ACAT1 Polyclonal Antibody | anti-ACAT1 antibody

Rabbit Polyclonal (IgG) to Human ACAT1

Cat. Num. AAA242905

• Gene Names: ACAT1; T2; MAT; ACAT; THIL
• Reactivity: Human, Mouse, Pig, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: ACAT1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human ACAT1; Anti-ACAT1 Antibody IHC-plus; ACAT; Acetoacetyl-CoA thiolase; Acetyl-CoA acetyltransferase 1; MAT; T2; THIL; Human ACAT1; anti-ACAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Pig, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This Polyclonal antibody is directed against human ACAT1 protein. The product was purified from serum by protein A chromatography. Cross-reactivity with ACAT1 from other sources has not been determined.
• Purity/Purification: Affinity Purified
• Form/Format: PBS, pH 7.4, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 427

• Applicable Applications for anti-ACAT1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (2.5 ug/ml), WB (1:1000 - 1:4000)
• Target Species: Human
• Immunogen Description: Synthetic peptide
• Immunogen Type: Synthetic peptide
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ACAT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Related Product Information for anti-ACAT1 antibody

The ACAT1 mRNA encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The ACAT gene spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

NCBI and Uniprot Product Information

• NCBI GI #: 4557237
• NCBI GeneID: 38
• NCBI Accession #: NP_000010.1
• NCBI GenBank Nucleotide #: NM_000019.3
• UniProt Accession #: P24752; Q96FG8; B2R6H1; G3XAB4
• Molecular Weight: 17,175 Da
• NCBI Official Full Name: acetyl-CoA acetyltransferase, mitochondrial
• NCBI Official Synonym Full Names: acetyl-CoA acetyltransferase 1
• NCBI Official Symbol: ACAT1
• NCBI Official Synonym Symbols: T2; MAT; ACAT; THIL
• NCBI Protein Information: acetyl-CoA acetyltransferase, mitochondrial; acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase
• UniProt Protein Name: Acetyl-CoA acetyltransferase, mitochondrial
• UniProt Gene Name: ACAT1
• UniProt Synonym Gene Names: ACAT; MAT
• UniProt Entry Name: THIL_HUMAN

NCBI Description

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Uniprot Description

ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Amino Acid Metabolism - tryptophan; Acetyltransferase; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - butanoate; EC 2.3.1.9; Mitochondrial; Amino Acid Metabolism - lysine degradation; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: enzyme binding; protein homodimerization activity; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding

Biological Process: response to starvation; response to hormone stimulus; ketone body catabolic process; ketone body biosynthetic process; branched chain family amino acid catabolic process; ketone body metabolic process; brain development; cellular lipid metabolic process; liver development; response to organic cyclic substance; protein homooligomerization

Disease: Alpha-methylacetoacetic Aciduria