CD1B / CD1A recombinant protein

Recombinant Human CD1B / CD1A Protein (Fc tag)

Cat. Num. AAA2545656

• Gene Names: CD1B; R1; CD1; CD1A
• Purity: > 88 % as determined by SDS-PAGE
• Synonyms: CD1B / CD1A; Recombinant Human CD1B / CD1A Protein (Fc tag); CD1B; CD1B / CD1A recombinant protein

• Host: Human Cells
• Purity/Purification: > 88 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 333

• Application Notes: The recombinant human CD1B /Fc is a disulfide-linked homodimer. The reduced monomer comprises 527 amino acids and has a predicted molecular mass of 58.6 kDa. The apparent molecular mass of the protein is approximately 59-69 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD1B / CD1A recombinant protein

Background: CD1B contains 1 Ig-like (immunoglobulin-like) domain and belongs to the CD1 family. CD1 family members are transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. During protein synthesis and maturation, they bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1B localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.. It is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. CD1B is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Description: A DNA sequence encoding the human CD1B (NP_001755) (Met1-Ser 303) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4502645
• NCBI GeneID: 910
• NCBI Accession #: NP_001755
• NCBI GenBank Nucleotide #: NM_001764.2
• UniProt Accession #: P29016; Q5TDK9; Q5TDL0; Q9UMM2
• Molecular Weight: 30,740 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD1b
• NCBI Official Synonym Full Names: CD1b molecule
• NCBI Official Symbol: CD1B
• NCBI Official Synonym Symbols: R1; CD1; CD1A
• NCBI Protein Information: T-cell surface glycoprotein CD1b
• UniProt Protein Name: T-cell surface glycoprotein CD1b
• UniProt Gene Name: CD1B

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. [provided by RefSeq, Jul 2008]

Uniprot Description

CD1B: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell surface; plasma membrane

Molecular Function: beta-2-microglobulin binding; endogenous lipid antigen binding; exogenous lipid antigen binding; protein binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; regulation of immune response