Rabbit anti-Human, Mouse ACAP1 Polyclonal Antibody | anti-ACAP1 antibody

ACAP1 Rabbit pAb

Cat. Num. AAA4759142

• Gene Names: ACAP1; CENTB1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: ACAP1; Polyclonal Antibody; ACAP1 Rabbit pAb; ArfGAP with coiled-coil; ankyrin repeat and PH domains 1; CENTB1; anti-ACAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.7mg/ml (varies by lot)

• Applicable Applications for anti-ACAP1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000; IHC-P: 1:25-1:100
• Immunogen: Fusion protein of human ACAP1
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of ACAP1 in Mouse lung tissue lysate using ACAP1 Rabbit pAb at dilution 1/450)

Immunohistochemistry (IHC)

(Immunohistochemistry of ACAP1 in paraffin-embedded Human thyroid cancer tissue using ACAP1 Rabbit pAb at dilution 1/25. Predicted cell location: Cytoplasm)

Related Product Information for anti-ACAP1 antibody

ACAP1, also designated Centaurin-? 1 (CENTB1 or Cnt-?1), is a member of the ADP ribosylation factor family of ARF6 GTPase-activating proteins (GAP). GAPs are important regulators of Arf function by controlling the return of ARF to its inactive state. ACAP1 is related to AGAP1 and ASAP1, and all three proteins are similarly expressed in fibroblast cells such as NIH/3T3. Internalization and recycling of integrin receptors is important in cell adhesion and migration modulation, and once inside a cell, proteins and membranes are transported to the endosome where they are sorted for recycling or degradation. ACAP1 promotes cargo sorting by associating directly to recycling cargo proteins. Preventing this interaction inhibits protein recycling. ACAP1 binds transferrin receptors, promoting their transport to the plasma membrane from the endosome. Akt induced phosphorylation of ACAP1 at Ser 554 regulates ACAP1 interaction to integrin in endosomes, and downregulation of Akt or ACAP1 may inhibit cell migration on Fibronectin.

NCBI and Uniprot Product Information

• NCBI GI #: 7661880
• NCBI GeneID: 9744
• NCBI Accession #: NP_055531.1
• NCBI GenBank Nucleotide #: NM_014716.3
• UniProt Accession #: Q15027; Q53XN9
• Molecular Weight: 81,536 Da
• NCBI Official Full Name: arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
• NCBI Official Synonym Full Names: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• NCBI Official Symbol: ACAP1
• NCBI Official Synonym Symbols: CENTB1
• NCBI Protein Information: arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; cnt-b1; centaurin-beta-1; centaurin, beta 1; Arf GAP with coiled coil, ANK repeat and PH domains 1
• UniProt Protein Name: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
• Protein Family: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ACAP1
• UniProt Synonym Gene Names: CENTB1; KIAA0050; Cnt-b1
• UniProt Entry Name: ACAP1_HUMAN

Uniprot Description

CENTB1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.

Protein type: Motility/polarity/chemotaxis; GAPs, ARF; GAPs

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; recycling endosome membrane

Molecular Function: zinc ion binding

Biological Process: protein transport; positive regulation of GTPase activity