Rabbit anti-Human ACAD11 Polyclonal Antibody | anti-ACAD11 antibody

ACAD11 Antibody

Cat. Num. AAA9412737

• Gene Names: ACAD11; ACAD-11
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: ACAD11; Polyclonal Antibody; ACAD11 Antibody; ACAD-11; anti-ACAD11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ACAD11 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 0.7 mg/ml (varies by lot)
• Sequence Length: 780

• Applicable Applications for anti-ACAD11 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:200-1:1000; Immunohistochemistry: 1:15-1:50
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to a region derived from internal residues of human Acyl-CoA dehydrogenase family, member 11
• Target Name: ACAD11
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 8%SDS-PAGE Lysates (from left to right): Human fetal liver tissue Amount of lysate: 40ug per lane Primary antibody: 1/300 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/15.)

Related Product Information for anti-ACAD11 antibody

The deduced full-length ACAD11 protein contains an N-terminal aminoglycoside phosphotransferase domain, followed by a mitochondria localization signal and ACAD N-terminal, middle, and C-terminal domains. It also has a glycosylation site and a C-terminal peroxisome-targeting signal. The ACAD domain contains a catalytic aspartate rather than the catalytic glutamate found in most other ACADs. Real-time RT-PCR detected variable ACAD11 expression in all tissues examined, with highest expression in adult brain, followed by heart, liver, spinal cord, and kidney. Western blot analysis of fractionated human cerebellum and kidney revealed thatACAD11 associated with mitochondrial membranes, but not mitochondrial matrix. Immunohistochemical analysis showed that ACAD11colocalized with a mitochondrial marker in human neuroblastoma cells.

Product Categories/Family for anti-ACAD11 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 38505218
• NCBI GeneID: 84129
• NCBI Accession #: NP_115545.3
• NCBI GenBank Nucleotide #: NM_032169.4
• UniProt Accession #: Q709F0; Q08AF0; Q658N9; Q658Y2; Q6ZND2; Q8WUT6; Q9H9R3
• Molecular Weight: 73,920 Da
• NCBI Official Full Name: acyl-CoA dehydrogenase family member 11
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase family, member 11
• NCBI Official Symbol: ACAD11
• NCBI Official Synonym Symbols: ACAD-11
• NCBI Protein Information: acyl-CoA dehydrogenase family member 11
• UniProt Protein Name: Acyl-CoA dehydrogenase family member 11
• UniProt Gene Name: ACAD11
• UniProt Synonym Gene Names: ACAD-11
• UniProt Entry Name: ACD11_HUMAN

NCBI Description

This gene encodes an acyl-CoA dehydrogenase enzyme with a preference for carbon chain lengths between 20 and 26. Naturally occurring read-through transcription occurs between the upstream gene NPHP3 (nephronophthisis 3 (adolescent)) and this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

ACAD11: Acyl-CoA dehydrogenase, that exhibits maximal activity towards saturated C22-CoA. Belongs to the acyl-CoA dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.3.99.-; Mitochondrial

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: mitochondrial membrane; peroxisome; nucleus

Molecular Function: FAD binding; transferase activity, transferring phosphorus-containing groups; very-long-chain-acyl-CoA dehydrogenase activity; long-chain-acyl-CoA dehydrogenase activity

Biological Process: fatty acid beta-oxidation using acyl-CoA dehydrogenase