Rabbit anti-Human, Mouse ABIN3 Polyclonal Antibody | anti-ABIN3 antibody

ABIN3 Antibody

Cat. Num. AAA9414579

• Gene Names: TNIP3; LIND; ABIN-3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: ABIN3; Polyclonal Antibody; ABIN3 Antibody; A20-binding inhibitor of NF-kappaB activation 3; TNFAIP3 interacting protein 3; TNIP3; Listeria-induced gene protein; LIND; anti-ABIN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 325

• Applicable Applications for anti-ABIN3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide near the carboxy terminus of human ABIN3.
• Target Name: ABIN3
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ABIN3 in mouse spleen tissue with ABIN3 antibody at 5 ug/mL.)

Related Product Information for anti-ABIN3 antibody

The nuclear factor NF-kB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-kB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-kB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-kB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-kB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-kB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.

Product Categories/Family for anti-ABIN3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 192807305
• NCBI GeneID: 79931
• NCBI Accession #: NP_079149
• NCBI GenBank Nucleotide #: NM_024873.5
• Molecular Weight: 44,886 Da
• NCBI Official Full Name: TNFAIP3-interacting protein 3 isoform 1
• NCBI Official Synonym Full Names: TNFAIP3 interacting protein 3
• NCBI Official Symbol: TNIP3
• NCBI Official Synonym Symbols: LIND; ABIN-3
• NCBI Protein Information: TNFAIP3-interacting protein 3
• UniProt Protein Name: TNFAIP3-interacting protein 3
• UniProt Gene Name: TNIP3
• UniProt Synonym Gene Names: ABIN-3
• UniProt Entry Name: TNIP3_HUMAN

Uniprot Description

TNIP3: Binds to zinc finger protein TNFAIP3 and inhibits NF- kappa-B activation induced by tumor necrosis factor, Toll-like receptor 4 (TLR4), interleukin-1 and 12-O-tetradecanoylphorbol-13- acetate. Overexpression inhibits NF-kappa-B-dependent gene expression in response to lipopolysaccharide at a level downstream of TRAF6 and upstream of IKBKB. NF-kappa-B inhibition is independent of TNFAIP3 binding.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 4q27

Molecular Function: polyubiquitin binding

Biological Process: MyD88-independent toll-like receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; toll-like receptor 4 signaling pathway